ShEEP Request for BZX Fluorescence Microscope

该提案要求Keyence BZ-X810荧光显微镜作为公共资源 共享核心实验室设施的主要设备，供生物医学研究人员使用 詹姆斯·A·黑利退伍军人医院(JAHVH)。JAHVH的生物医学研究由 著名的、资金雄厚的调查人员。拟议的工具将在12年前主要使用 调查人员，包括2名高级研究职业科学家和2名研究职业科学家 与阿尔茨海默氏症、癌症、糖尿病、肥胖、海湾等相关的正在进行的研究项目 战争疾病、呼吸道合胞疾病、创伤性脑损伤和再生医学。在……里面 此外，这些人还拥有其他补助金(NIH、AHA、FLDOH、DoD等)。这将利用 荧光显微镜有助于在现有和新的研究项目的基础上发展，这些项目将受益 退伍军人。 这次收购将是对15岁以上已不存在的公司的迫切需要的替代 目前可以使用的显微镜。没有其他多功能荧光显微镜是 在JAHVH内提供，在南佛罗里达大学(USF，我们的 附属大学)在美国联邦内需求量很大，由5所学院共享，通常时间段是 不可用，因为退伍军人管理局的研究人员没有优先于USF的研究人员。另外， 运输到美国联邦会造成延误，对样品产生不利影响。 这款荧光显微镜有很多优点：体积紧凑，节省桌面空间；方便 为所有用户提供操作；敏感光学提供高质量的结果；指定测量范围； 自动批量捕获所需图像；统一条件下的高含量分析； 采用高分辨率拼接和Z堆叠的多维图像捕获；结构化 照明消除了荧光模糊，只需一次点击即可提供清晰的图像；捕获 通过记录其最外层位置的坐标来自动获取整个样本； 准确分析结构和3D信号。 多功能荧光显微镜以高分辨率和高分辨率采集图像 与常规显微镜相比，它具有更高的灵敏度。多功能一体机，暗室 是不需要的。它可以同时扫描多张幻灯片。这台显微镜的其他优点 包括增强信噪比、对厚样品进行可理解和仔细检查， 高质量的图像是研究项目的关键，也是高质量出版物的核心 影响期刊和获得联邦资金。此外，仪器将加速和 提高JAHVH研究人员的研究效率，为开发新的 研究方向，增加调查人员之间的协同，增加翻译 JAHVH研究方案的研究能力。 总体而言，拟议的工具将显著影响和加强VAMC的使命，以 通过允许退伍军人研究人员进行尖端技术来促进退伍军人的医疗保健 基础、翻译或临床领域的研究。这些努力将大大有助于 JAHVH的长期研究目标。

The proposal requests KEYENCE BZ-X810 Fluorescence Microscope as a common resource shared major equipment integral to the core lab facility to be used by biomedical researchers of the James A Haley Veterans Hospital (JAHVH). Biomedical research at JAHVH is conducted by renowned, well-funded investigators. The proposed instrument will be predominantly used by 12 investigators, including 2 Senior Research Career Scientists and 2 Research Career Scientists with ongoing research programs related to Alzheimer's Disease, cancer, diabetes, obesity, Gulf War Illness, respiratory syncytial disease, traumatic brain injury and regenerative medicine. In addition, these individuals have other grants (NIH, AHA, FLDOH, DoD, etc.) that will utilize the fluorescence microscope to help build on existing and new research projects that will benefit Veterans. This acquisition will be a much-needed replacement for the greater than 15-year- old defunct microscope presently available for use. No other multi-functional fluorescence microscopes are available within JAHVH and the one available at the University of South Florida (USF, our affiliate university) is in high-demand within USF, shared by 5 colleges and often time-slots are unavailable as VA researchers are not given preference over USF researchers. Additionally, transportation to USF causes delays adversely affecting samples. This fluorescence microscope has a lot of benefits: Compact size saves benchtop space; Easy operation for all users; Sensitive optics deliver high-quality results; Specify measurement range; Automatically batch capture desired images; High-content analysis with uniform conditions; multi-dimensional image capture with-high-resolution-stitching and Z-stacking; Structured illumination eliminates fluorescence blurring and delivers clear images in just one click; Capture an entire specimen automatically by registering the coordinates of its outermost positions; Accurate analysis of structure and 3D signals. The multi-functional fluorescence microscope captures images at a higher resolution with high sensitivity compared to conventional microscope. Multi-function is built all-in-one, the dark room is not needed. It can scan multi-slides at the same time. Other benefits of this microscope include enhanced signal-to-noise ratio, comprehensible and close examination of thick samples, High quality images are the crux of research projects and are central to publications in high impact journals and obtaining federal funding. Moreover, the instrumentation will accelerate and enhance the research productivity of the JAHVH investigators, allow for development of new research directions, increase synergy between investigators, and increase the translational research capacity of the JAHVH Research Program. Overall, the proposed instrument will significantly impact and augment VAMC's mission to advance the healthcare of Veterans by allowing VA researchers to conduct cutting-edge research in basic, translational or clinical fields. These endeavors will substantially contribute to the long-term research goals of the JAHVH.