ShEEP Request for Acquisition a 10x Genomics Single Cell RNA Sequencer

项目总结/摘要 单细胞转录组学技术是一种新的和必要的技术，它允许直接测量细胞内的转录水平。 在单细胞水平上的基因表达，以定量细胞群体的细胞内异质性并表征细胞 表型，细胞信号传导途径和功能，以及细胞间的动态细胞转换。这提供了一 单细胞水平的分析，从而提供了以前无法获得的生物学见解， 而且成本大大降低。10 x单细胞RNAseq（scRNA-seq）铬单细胞基因表达 控制器提供了一种无与伦比的方法，用于揭示细胞间基因表达的变异性， 鉴定来源于组织或细胞的异质样品中的稀有细胞类型。这个单细胞测序 该平台将使VA研究人员能够超越传统的全球标记基因分析， 类型或细胞功能状态以及伴随的调控途径中的动态基因变化。scRNA-seq 现在广泛用于寻求解决以前认识不足的细胞异质性的研究， 细胞发育和分化的关键过程，阐明造血的关键途径， 了解预测免疫功能的基因调控网络。单细胞的静态快照 转录组可以为分化和激活状态的各个阶段提供有力的窗口， 它们在小区之间很少同步。重要的是，发育过程中复杂的生物过程 生物学、癌症、神经科学、免疫学和传染病通常涉及多个单独的细胞， 有不同的命运状态和功能这些现在可以在细胞中表征、捕获和解析。 与单个scRNA-seq实验水平相同。这个带有铬单细胞基因的单细胞RNA测序仪 表达解决方案将提供一个无与伦比的资源，为所有的VA研究调查员在我们的VA 大洛杉矶医疗保健系统医疗中心。10 x单细胞RNAseq将支持以下功能 VA资助的Merit Review和职业发展研究项目的最终目标是改善和 帮助退伍军人在我们的机构医疗保健：1.神经再生的治疗，如恢复神经 中风或脊髓损伤后的功能，2.甲状腺功能减退症，3。微生物调节食欲，肥胖， 代谢综合征、糖尿病和脂肪肝，4.前列腺癌5例阿尔茨海默病，6。钩端螺旋体病，7. 心室切除术后心肌再生，8。结肠对应激反应的调节和 炎症，9.肠上皮细胞增殖和通透性，10。调节肠道运动功能，以及 内脏疼痛改变了各种压力，11。肠道细菌代谢物，12.帕金森氏症，13。结肠 癌

Project Summary/Abstract Single cell transcriptomic technology is a new and essential technology that allows for the direct measurement of gene expression at the single cell level to quantify cell population intracellular heterogeneity and characterize cell phenotypes, cell signaling pathways and functions, and dynamic cellular transitions cell by cell. This provides an analysis at the single cell level thus providing insights into biology that have been previously inaccessible and also at a greatly reduced cost. The 10x Single Cell RNAseq (scRNA-seq) Chromium Single Cell Gene Expression Controller provides an unparalleled approach for uncovering cell-to-cell gene expression variability and identifying rare cell types in heterogeneous samples derived from tissue or cells. This single cell sequencing platform will allow VA researchers to extend beyond traditional global marker gene analysis to characterize cell types or cell functional states and the concomitant dynamic gene changes in regulatory pathways. scRNA-seq is now widely employed in studies seeking to resolve previously under-recognized cellular heterogeneity, define key processes in cell development and differentiation, unravel critical pathways of hematopoiesis, and understand the gene regulatory networks that predict immune function. A static snapshot of single-cell transcriptomes can provide a powerful window onto the various stages of differentiation and activation states, which are rarely synchronized between cells. Importantly, complex biological processes in developmental biology, cancer, neuroscience, immunology, and infectious disease usually involve multiple individual cells that have different fates, states, and functions. These can be now characterized, captured and resolved at the cellular level with a single scRNA-seq experiment. This single cell RNA sequencer with the Chromium Single Cell Gene Expression Solution will provide an unparalleled resource for all of the VA research investigators at our VA Medical Center Greater Los Angeles Healthcare System. The 10x Single Cell RNAseq will support the following VA funded Merit Review and Career Development research projects that have the ultimate goal to improve and help Veterans healthcare at our institution: 1. Treatments for neural regeneration, such as restoring neural function after stroke or spinal cord injury, 2. Hypothyroidism, 3. Microbial regulation of appetite, obesity, metabolic syndrome, diabetes and fatty liver, 4. Prostate cancer, 5. Alzheimer disease, 6. Leptospirosis, 7. Regenerating myocardium after ventricular resection, 8. Modulation of colonic response to stress and inflammation, 9. Intestinal epithelial cell proliferation and permeability, 10. Regulation of gut motor function, and visceral pain altered by various stressors, 11. Gut bacterial metabolites, 12. Parkinson’s disease, and 13. Colon cancer.