ShEEP Request for Single-Cell Next-Generation Sequencing Library Preparation System

这只绵羊需要一种单细胞下一代测序文库制备系统 申请，包括1)10倍基因组铬控制器和培训及设置试剂；2)a 用于质量控制的Thermo-Fisher Countess II自动细胞计数器；以及3)用于下游的工作站 管道数据的处理和分析。正在从SHEEP应用程序请求项1，项2和3 将由旧金山退伍军人医疗保健系统的研究和开发部提供。 组装好的系统将与目前的Illumina NextSeq 500测序仪集成在分子 核心，并提供对单细胞下一代测序项目的先进技术的访问，包括 单细胞水平的转录组分析、基因突变筛选和表观基因组分析。 该系统有四个主要用户和三个次要用户，调查1)癌症异质性和 药物治疗反应(主要使用者，Chris Lau)；2)心力衰竭和药物治疗反应(主要使用者， 3)动脉粥样硬化和心血管疾病中代谢和炎症的相关性 (主要使用者，Robert Raffai)；4)肺动脉高压和右心衰竭的机制 (主要使用者，Anthony Baker)；5)创伤性脑损伤中的神经营养素受体调节剂 阿尔茨海默病(小使用者，Stephen Massa)；6)氧化应激中的线粒体结构和功能- 相关疾病(小使用者，Gary Cecchini)；以及7)前列腺癌的表观基因组变化(小使用者， 田中雄一郎)。单细胞下一代测序文库准备系统的可用性将 为这些研究人员提供手段，以对各自疾病的机制进行研究，确定 翻译应用于诊断、预后和治疗策略的特定靶点，从而 建立退役军人精准医疗和个性化医疗服务体系。

A single-cell next-generation sequencing library preparation system is being requested in this ShEEP application, which consists of 1) 10X Genomics Chromium Controller and training and set up reagents; 2) a Thermo-Fisher Countess II Automated Cell Counter for quality control; and 3) a Workstation for downstream pipeline data processing and analysis. Items 1 is being requested from the ShEEP application, items 2 and 3 will be provided by the Research and Development Department at the San Francisco VA Health Care System. The assembled system will be integrated with the current Illumina NextSeq 500 sequencer at the Molecular Core and provide access to advanced technology of single cell next generation sequencing projects, including transcriptome analysis, genetic mutation screening and epigenome analysis, at single cell level. There are four Major Users and three Minor Users for this system, investigating 1) cancer heterogeneity and drug treatment responses (Major User, Chris Lau); 2) heart failure and drug treatment responses (Major User, Paul Simpson); 3) correlation of metabolism and inflammation in atherosclerosis and cardiovascular disease (Major User, Robert Raffai); 4) mechanisms of pulmonary arterial hypertension and right ventricular failure (Major User, Anthony Baker); 5) neurotrophin receptor modulation agents in traumatic brain injury and Alzheimer’s disease (Minor User, Stephen Massa); 6) mitochondrial structure and functions in oxidative stress- related diseases (Minor User, Gary Cecchini); and 7) epigenome changes in prostate cancer (Minor User, Yuichiro Tanaka). The availability of the single-cell next-generation sequencing library preparation system will provide means for these investigators to conduct research on the mechanisms of respective diseases, identify specific targets for translational applications in diagnosis, prognosis and therapeutic strategies, thereby establishing the precision medicine and personalized health care delivery for Veterans.