INTERNATIONAL WORKSHOP ON HUMAN Y CHROMOSOME

人类Y染色体是人类基因组中最小的染色体， 含有约30 Mb的常染色质。因为它的基因简单， 实际信息序列可以小至10 - 14 Mb， 估计含有约200 - 350个功能基因。这些基因中的大多数 要么与雄性生理学有关，要么是多基因性状的成员。 最近，第一届人类Y染色体图谱国际研讨会 于1994年4月2 - 5日在英国剑桥的休斯厅举行。这 来自8个国家的64名科学家参加了讲习班， 来自美国的参与者。许多目标已经实现 通过剑桥研讨会，包括达成共识 整个常染色质的缺失和物理重叠群图谱， 亚区，14个基因的定位和许多新的STS在这些地图上， X-Y同源性的定义，人和小鼠Y染色体的比较定位 染色体和EST定位克隆策略的评价 从这条染色体上收集。尽管取得了这些进展， 需要达到确定完整基因的最终目标， 这条染色体的信息。首先，许多区域删除和 YAC中的物理重叠群图谱、PI克隆和互补序列必须 结合到整个物理重叠群图谱中，从而构建 高分辨率常染色质物理图谱。证明了这 对大规模定位克隆技术的巨大兴趣， 正在分离更多的cDNA和捕获的外显子EST 在许多实验室里从这条染色体中分离出来。这些EST将需要 整合到人Y染色体的第一代转录图谱中， 染色体 基于上述发展，我们建议进行第二次 人类Y染色体国际研讨会九月十七日举行 1995年20日在加州太平洋格罗夫的阿西洛马会议中心举行。这 研讨会将重点放在建设共识删除和物理 整个常染色质及其亚区的重叠群图谱， X-Y同源性和比较映射，以及第一个- 人类Y染色体转录图谱的生成。这将是一 这是该领域调查人员聚集和 提出他们的结果，调和和讨论他们的分歧， 研究结果，并相互交流和合作， 共同利益拟议的讲习班将是一个重要的里程碑 最终目标是完全确定 人类Y染色体上的基因。

The human Y chromosome is the smallest chromosome in the human genome and contains about 30 Mb of euchromatin. Because of its genetic simplicity, the actual informative sequence may be as little as 10-14 Mb and has been estimated to contain about 200-350 functional genes. Most of these genes are either related to male physiology or members of polygenic traits. Recently the First International Workshop on Human Y Chromosome Mapping was held on April 2-5,1994 at Hughes Hall, Cambridge, England. This workshop was attended by 64 scientists from 8 countries, including 15 participants from the United States. Numerous goals have been achieved through the Cambridge Workshop, including the generation of consensus deletion and physical contig maps of the entire euchromatin and subregions, localization of 14 genes and many new STSs on these maps, definition of X-Y homology, comparative mapping of human and mouse Y chromosomes and evaluation of positional cloning strategies for EST collection from this chromosome. Despite these advances, more work is needed to achieve the ultimate goal of determining the complete genetic information of this chromosome. First, many of the regional deletion and physical contig maps in YACs, PI clones and cosmids have to be incorporated into the overall physical contig map, thereby constructing a higher resolution physical map of the euchromatin. As evidenced by the tremendous interests in large-scale positional cloning techniques, additional ESTs in terms of cDNAs and trapped exons are being isolated from this chromosome in many laboratories. These ESTs will needed to be incorporated into a first generation transcription map of the human Y chromosome. Based on the above developments, we propose to conduct a Second International Workshop on Human Y Chromosome to be held on September 17- 20,1995 at the Asilomar Conference Center, Pacific Grove, California. This workshop will focus on the construction of consensus deletion and physical contig maps of the entire euchromatin and the subregions, on advances of X-Y homology and comparative mappings, and the assembly of a first- generation transcription map of the human Y chromosome. It will be an important opportunity for investigators in this field to assemble and present their results, reconcile and discuss about differences in their findings, and interact and collaborate with each others in studies of mutual interests. The proposed workshop will be an important landmark towards the ultimate goal of a complete determination of the genetic content on the human Y chromosome.