ShEEP Request for IVIS SPECTRUMCT, 2D and 3D Optical In Vivo Tomography System.

项目摘要/摘要 我们建议收购IVIS(活体成像系统)SPECTRUMCT。它有多个 功能，包括2D生物发光、3D生物发光/荧光断层扫描、PET、 对于小动物和大动物的CT和MRI功能，检测体内的钙离子内流即可 用于疼痛研究和药物毒性评估，这对于识别和开发新的 毒品。目前还没有这种高灵敏度的层析光学成像系统 提供给杰西·布朗VAMC的研究人员，以支持退伍军人管理局资助的研究。这台仪器将会 成为Jesse Brown VAMC的第一个IVIS SPECTRUMCT，并将显著加速数据 通过提供复杂的体内分析功能，为退伍军人管理局资助的研究生成 目前在杰西·布朗VAMC或附近的大学无法使用。通过使用新的 分析活体成像能力，我们还希望使用更少的动物来进行我们的 建议的研究，取消需要牺牲动物的时间进程研究，然后 组织采集。退伍军人管理局资助的这些机构将利用大约85%的仪器时间 主要用户，剩余时间将用于支持NIH、国防部资助或其他试点项目 项目。该仪器将放置在退伍军人管理局动物设施中，退伍军人管理局调查人员可以在那里 在不将动物带出动物设施的情况下进行体内成像分析，即 专为无特定病原体的动物而设计。按主修/辅修调取乐器 用户将通过任命的咨询委员会的行政监督得到保障 由研发委员会主席主持。行政/技术服务将由Dr. 桑本拥有十多年的成像分析经验，并由协调员Dr。 Natarajan Anbazhagan，拥有4年以上直接操作IVIS的经验 SPECTRUMCT系统。确保设备安全、适当地运行 保持最高效率，桑本博士和纳塔拉扬·安巴扎根博士将进一步 由Perkin Elmer技术团队通过强化培训课程进行培训。

Project Summary/Abstract We propose to acquire an IVIS (IN VIVO IMAGING SYSTEM) SPECTRUMCT. It has multiple functions, including 2D Bioluminescence, 3D Bioluminescence/ Fluorescence Tomography, PET, CT and MRI functions for small and large animals, detection of in vivo Ca2+ influx that can be useful for pain study, and drug toxicity evaluation that is critical for identifying and developing new drugs. Presently there is no any highly sensitive tomographic optical imaging systems of this kind available to researchers in Jesse Brown VAMC to support VA-funded studies. This instrument will be the first IVIS SPECTRUMCT at the Jesse Brown VAMC, and will significantly accelerate data generation for the VA-funded studies by providing sophisticated in vivo analytical capabilities that are currently unavailable at the Jesse Brown VAMC or nearby universities. By using the new analytical in vivo imaging capabilities, we also expect to use significantly fewer animals for our proposed studies by eliminating time-course studies that require sacrificing animals followed by tissue harvest. Approximately 85% of the instrument time will be utilized by these VA-funded major users, and the remaining time will be used to support NIH-, DoD-funded or other pilot projects. This instrument will be located in the VA Animal Facility where the VA investigators can conduct in vivo imaging analyses without taking the animals out of the animal facility, which is designed to house specific pathogen free animals. Access to the instrument by the major/minor users will be ensured through the administrative oversight of an Advisory Committee appointed by the Chair of the R&D Committee. Administrative/technical service will be provided by Dr. Sampen who has imaging analyses experience for more than a decade, and by a coordinator, Dr. Natarajan Anbazhagan who has over 4 years of experience in directly operating the IVIS SPECTRUMCT system. To ensure that the equipment is operated safely and appropriately maintained with maximum efficiency, Drs. Sampen and Natarajan Anbazhagan will be further trained by the Perkin Elmer Technical team with intensive training courses.