Project 2: Capsid dynamics and interactions with host factors

P2.摘要 在这个项目中，我们将测试我们的假设，建立通过广泛的研究，在PCHPI使用多种 技术，动力学是决定衣壳组装，成熟和相互作用的重要因素 与宿主蛋白质，如TRIM 5 α。具体来说，我们将i）确定构象动力学在CA中的作用 ii）确定衣壳/TRIM 5 α动力学并描绘原子上的分子间界面 iii）确定衣壳/CypA的动力学和CypA诱导的结构扰动 与来自不同菌株的CA组装体的结合表现出衣壳稳定性的差异。完成 本项目的具体目标，我们将采用多管齐下的方法，包括MAS NMR，冷冻EM，MD 模拟，以及病毒学和分子生物学方法。我们将联合收割机 PCHPI的研究人员和合作者，并密切合作，以解决多学科的问题 关于HIV-1感染周期中动力学的作用。

P2. Abstract In this project, we will test our hypothesis, established through extensive studies at the PCHPI using multiple techniques, that dynamics are an important factor determining capsid assembly, maturation, and interactions with host proteins, such as TRIM5α. Specifically, we will i) determine the role of conformational dynamics in CA assemblies; ii) determine the capsid/TRIM5α dynamics and delineate the intermolecular interfaces at atomic resolution; and iii) determine the dynamics of capsid/CypA and structural perturbations induced by CypA binding to CA assemblies from different strains exhibiting differences in capsid stability. To accomplish the specific aims of this project, we will employ a multi-pronged approach including MAS NMR, cryo-EM, MD simulations, as well as virology and molecular biology approaches. We will combine the complementary expertise of the PCHPI investigators and collaborators and work in close synergy to tackle the multi-disciplinary questions concerning the role of dynamics in the HIV-1 infection cycle.