Center for Advanced Renal Microscopic Analysis

摘要(中心概览) 印第安纳大学在过去的二十年里开发了一种独特的、高度互动的、综合的和 由医生、基础科学家、计算机科学家、工程师和训练有素的 致力于技术支持的团队，以尖端设备和技术为动力，开发和利用 活体光学显微镜和肾脏的三维定量数字图像分析。这个科学中心有 导致了一个高效率的肾病学部门，具有广泛的互动，涉及到 伊利诺伊州医学院校区、普渡大学、印第安纳大学布鲁明顿分校和 更远一点。特别是，现有的奥布莱恩高级肾脏显微分析中心已经允许我们的 来自世界各地的生物医学基地和外部用户受益于科学专业知识和设备 可在成像中心购买。专业技术的开发和基于用户的定制 显微镜和定量分析一直是现有P-30中心的标志。因此，主要的 本建议的目的是继续提供并进一步发展量化的综合方法 活体显微镜和组织显微镜促进分子、细胞和遗传学方法研究疾病 为我们的生物医学基地和来自世界各地的研究人员提供技术和治疗方法。我们已经添加了 一种定量3D组织成像核心，以满足对专业知识和基础设施的需求 支持大规模、高含量、高分辨率显微镜的收集和定量分析 数据。通过这样做，该中心将为肾脏/泌尿外科研究人员提供一套新的研究方法， 其他地方没有的定量分析工具和荧光生物传感器或探针，这些工具将有助于 肾脏和泌尿系统疾病的生物医学研究、药物发现和治疗方法。

Abstract (Center Overview) Indiana University has over the last twenty years developed a unique, highly interactive, integrated and synergistic team of physicians, basic scientists, computer scientists, engineers and a highly trained and committed technical support team, powered by cutting-edge equipment and technolog, to develop and utilize intravital optical microscopy and 3D quantitative digital image analysis of the kidney. This scientific hub has resulted in a highly productive Division of Nephrology with far-reaching interactions involving multiple investigators on the IU medical school campus, Purdue University, Indiana University- Bloomington and beyond. In particular, the existing O'Brien Center for Advanced Renal Microscopic Analysis has allowed our biomedical base and outside users from around the world to benefit from the scientific expertise and equipment available at the imaging center. The development and user-based customization of specialized techniques of microscopy and quantitative analysis have been a hallmark of the existing P-30 center. Therefore, the primary purpose of this proposal is to continue to offer and further develop an integrated approach to quantitative intravital and tissue microscopy to facilitate molecular, cellular and genetic approaches to the study of disease processes and their therapy to our biomedical base and investigators from around the world. We have added a quantitative 3D tissue imaging core to address the need for specialized expertise and infrastructure to support the collection and quantitative analysis of large-scale, high-content and high resolution microscopy data. In doing so, this Center will provide renal/urological researchers with a novel set of research methods, quantitative analysis tools and fluorescent biosensors or probes not available elsewhere, tools that will facilitate biomedical research, drug discovery, and therapeutic approaches to Kidney and Urologic diseases.