Regulation of the Oncogenic Stress Response in Helicobacter pylori-infected cells

描述(申请人提供)：胃腺癌(GC)仍然是最常见的癌症形式之一，也是全球癌症相关死亡的主要原因之一。全世界每年约有60万新的胃癌病例可归因于幽门螺杆菌感染，使这种细菌病原体成为胃恶性肿瘤的最大已知危险因素。然而，只有一定比例的感染者会患上肿瘤，这突显了定义调节细菌和感染者之间的致瘤相互作用的机制的重要性。我们提出了一个创新的假设，即在幽门螺杆菌的致瘤潜力和致癌应激反应途径(OSR)的失活之间建立了分子联系，OSR是防止胃细胞发生肿瘤转化的主要肿瘤抑制机制。这一假设得到了对感染幽门螺杆菌的人和动物的研究产生的强有力的初步数据的支持。我们的研究表明，幽门螺杆菌对OSR的抑制与胃癌的发生密切相关，而致癌的幽门螺杆菌菌株能够 以阻止OSR的监视。我们将在这些调查结果的基础上进一步调查OSR 幽门螺杆菌的抑制是胃腺癌发生的关键因素。 在目标1中，我们将定义幽门螺杆菌信号转导抑制的分子基础 OSR。目的2利用动物模型和胃组织类物质，研究OSR在体内对胃壁龛的调节作用。在目标3中，我们将表征细菌毒力因子的自然变异性如何影响OSR。我们还将测试在胃癌风险高和低的地理区域收集的人类临床标本和幽门螺杆菌临床分离株。综上所述，拟议的研究将为与幽门螺杆菌感染相关的肿瘤发生提供新的见解，研究幽门螺杆菌感染条件下OSR的调控。THI将有助于揭示胃肿瘤发展的潜在危险因素，并为将这些临床相关数据转化为幽门螺杆菌感染患者的新治疗应用奠定基础。

DESCRIPTION (provided by applicant): Gastric adenocarcinoma (GC) remains one of the most common forms of cancer and one of the leading causes of cancer-related death worldwide. Approximately six hundred thousand new cases of gastric cancer/year are attributable to Helicobacter pylori infection worldwide, making this bacterial pathogen the strongest known risk factor for gastric malignancy. However, only a percentage of infected individuals develop neoplasia, underscoring the importance of defining mechanisms that regulate tumorigenic interactions between bacteria and infected people. We have developed an innovative hypothesis that establishes a molecular link between the tumorigenic potential of H. pylori and inactivation of the Oncogenic Stress Response pathway (OSR), a major tumor suppressor mechanism that prevents tumorigenic transformation of gastric cells. This hypothesis is supported by strong preliminary data generated by studies of human individuals and animals infected with H. pylori. Our research revealed that suppression of the OSR by H. pylori is strongly linked to gastric carcinogenesis and that tumorigenic H. pylori strains are able to inhibit surveillance by the OSR. We will build on these findings to further investigate the OSR inhibition by H. pylori as a critical factor contributing to development of gastric adenocarcinoma. In aim 1, we will define the molecular underpinning of H. pylori signaling toward inhibition of the OSR. In aim 2, using the animal models and gastric organoids, we will investigate regulation of the OSR in the gastric niche in vivo. In aim 3, we will characterize how natural variability of bacterial virulence factors affects the OSR. We will also test human clinical specimens and H. pylori clinical isolates collected in geographical areas with high and low gastric cancer risk. Taken together, the proposed studies will provide novel insights into tumorigenesis associated with H. pylori infection, investigating the OSR regulation in condition of H. pylori infection. Thi will help to reveal the potential risk factors for gastric tumor development and lay the groundwork for translation of these clinically relevant data into novel therapeutic applications in H. pylori - infected patients.