Cell Isolation and Organ Function Core

心肺血管生物学(CPVB)生物医学研究中心的中心目标 优秀(Cobre)是理解血管疾病发病的基本机制并翻译 这些发现被用于心脏和肺部疾病的治疗。血管损伤、修复和重塑至关重要。 对于第二阶段项目中研究的疾病--支气管肺发育不良(BPD)的发病机制， 动脉粥样硬化、特发性肺纤维化、肺动脉高压和急性呼吸窘迫 综合征(ARDS)。损伤反应中的心脏发育和重塑同样包括适应性 和适应不良的反应。血管生成功能障碍，内皮对损伤的修复反应， 在ARDS、BPD、IPF和其他心脏和肺组织中可能发生异常的血管重构。 条件。此外，一些先天性心脏缺陷是由转录因子的突变引起的 调节心肌细胞和内皮细胞(EC)在发育过程中的功能。因此，增加了对 调节血管系统适应损伤和修复的机制可能导致独特的 阻断疾病进展或促进或加强心肺血管修复的方法。 在第一阶段，细胞/器官核心开发了用于细胞分离和器官功能的集中式系统 并为Cobre项目提供了可重复和准确的数据。其他服务 正在为罗德岛和第二阶段的血管生物学家提高细胞/器官核心能力 研究人员是：1)细胞分离的类型；2)免疫组织化学分析；3)内皮细胞功能 初步分离；iv)激光多普勒成像；以及v)体内血管生成。核心将提供关于以下方面的咨询 实验设计，提供尖端技术方法，生成和分析数据，并充当 为CPVB Cobre调查人员和罗德岛其他人提供的资源。核心还将发展一项业务 规划现有服务的财务稳定性，并确定新的业务或协作机会，以 确保CPVB核心的可持续性。细胞/器官核心的总体目标是促进科学 项目和试点调查员的目标和技术汇总表，具体目标如下。(1) 通过提供可重复性、经济性和高效性来提高CPVB Cobre研究项目的生产率 质量一)细胞分离、增殖、表型鉴定和功能评估二)评估 心、肺、血管结构和功能及血管生成；(Iii)体内瞬时基因操作。(2) 获取、建立和传播技术和仪器，以提供最先进的研究工具 在罗德岛进行血管生物学研究。(3)建立流程和程序，支持 细胞/器官核心的可持续性。CPVB Cobre细胞/器官核心将支持血管生物学 研究，包括新的调查人员、项目、技术方法和想法，以增强 罗德岛的研究。

The central goals of the CardioPulmonary Vascular Biology (CPVB) Center for Biomedical Research Excellence (COBRE) are to understand basic mechanisms of vascular disease pathogenesis and to translate these findings into treatments for heart and lung diseases. Vascular injury, repair, and remodeling are critical to the pathogenesis of the conditions studied in Phase II projects--bronchopulmonary dysplasia (BPD), atherosclerosis, idiopathic pulmonary fibrosis (IPF), pulmonary hypertension, and acute respiratory distress syndrome (ARDS). Cardiac development and remodeling in response to injury similarly include both adaptive and maladaptive responses. Dysfunction of angiogenesis, the reparative response of the endothelium to injury, and aberrant vascular remodeling have been proposed to occur in ARDS, BPD, IPF and other heart and lung conditions. In addition, some congenital heart defects result from mutations in transcription factors that regulate cardiomyocyte and endothelial cell (EC) function in development. Thus, an increased understanding of the mechanisms regulating the adaptation of the vasculature to injury and repair may lead to unique approaches to interrupt disease progression or to promote or enhance cardiopulmonary vascular repair. During Phase I the Cell/Organ Core developed centralized systems for cell isolations and organ function measurements and provided reproducible and accurate data for COBRE Projects. Additional services underway to enhance the Cell/Organ Core capabilities for the vascular biologists in Rhode Island and Phase II investigators are expansion of i) type of cell isolations; ii) immunohistochemical analyses; iii) EC function of primary isolates; iv) laser Doppler imaging; and v) in vivo angiogenesis. The Core will offer consultation on experimental design, provide cutting edge technological approaches, generate and analyze data, and serve as a resource for CPVB COBRE investigators and others in Rhode Island. The Core will also develop a business plan for financial stability of existing services and identify novel business or collaborative opportunities to ensure sustainability of the CPVB core. The overall goal of the Cell/Organ Core is to facilitate the scientific objectives and technical repertoire of the Project and Pilot Investigators by the following specific aims. (1) Enhance the productivity of CPVB COBRE research projects by offering reproducible, economical, and high quality i) cell isolation, propagation, phenotypic characterization and functional assessment ii) assessment of heart, lung, vessel structure and function and angiogenesis, and (iii) transient gene manipulation in vivo. (2) Acquire, establish, and disseminate technologies and instrumentation to provide state of the art research tools for vascular biology research in Rhode Island. (3) Establish processes and procedures that support the sustainability of the Cell/Organ Core. The CPVB COBRE Cell/Organ Core will support vascular biology research, including new investigators, projects, technical approaches and ideas that enhance the impact of research in Rhode Island.