Core C - Bone Core

核心C：骨芯摘要 该计划项目的核心将提供共享的设施和服务， 处理和解释来自临床样品和动物模型的组织， 方案的所有项目。这将通过以下目标实现： 目标1.实验设计/咨询。这包括有关端点的方法 分析和能力和方法的局限性。 目标2.病理学解释。对于组织学标本，组织病理学和 免疫组化评分。 目标3.组织处理。骨组织和汇集资源， 组织处理这包括固定，脱钙，石蜡包埋和切片 脱钙切片及固定、塑料包埋、不脱钙切片制片。 目标4。矿化组织的常规和特殊染色。这包括例如 矿化组织、破骨细胞和免疫组织化学。 目标5。骨切片和肿瘤组织的组织形态计量学分析。 这包括特定骨相关结构和细胞参数的测量。 目标6。临床组织样本的获取。骨髓样本 播散的肿瘤细胞分离和用于循环肿瘤细胞的血液以及需要的血清。 这些服务的核心支持将提高样本分析的效率， 通过类似的模型系统和共同的分析，项目之间的相互作用。一 作为一个团体，对该计划的重大好处将是一个标准化的格式，用于分析 将使用不同的实验方法， 方法/目标。这将提供有价值的信息，可以共享和比较 项目中的所有项目。为了实现这些目标，核心是由博士。 他是骨骼生物学专家。此外，核心由Dr. 委员会认证的泌尿肿瘤病理学家Rohit Mehra和泌尿科医生托德摩根博士 以及在播散性肿瘤细胞获取和生物学方面具有专长的医生科学家。 我们的顾问Tom Wronski博士提供了骨组织形态计量学方面的其他专业知识， 全国公认的专家在这一领域和骨病理学博士大卫卢卡斯，解剖 专门研究骨骼的病理学家总的来说，这个核心将继续提供全面的 以及本计划所有项目所需的独特服务。

CORE C: Bone Core Abstract The Bone Core of the Program Project will provide shared facilities and services for procuring, processing and interpretation of tissues from both clinical samples and animal models utilized in all projects of the program. This will be achieved through the following aims: Aim 1. Experimental design/consultation. This include methods regarding endpoint analyses and abilities and limitations of methods. Aim 2. Pathological interpretation. For histologic specimens, histopathology and scoring of immunohistochemistry. Aim 3. Processing of tissues. Both osseous tissues and pooling resources for soft tissue processing. This includes fixation, decalcification, paraffin embedding and sectioning of decalcified sections and fixation, plastic embedding and sectioning of undecalcified sections. Aim 4. Routine and special stains for mineralized tissues. This includes for mineralized tissues, osteoclasts and immunohistochemistry. Aim 5. Histomorphometric analysis of both osseous sections and tumor tissue. This includes measurement of specific bone-related structural and cellular parameters. Aim 6. Procurement of clinical tissue samples. Bone marrow samples for disseminated tumor cells isolation and blood for circulating tumor cells and serum as needed. Core support of these services will promote efficiency of specimen analysis and facilitate interactions between projects through the similar model systems and their common analyses. A significant benefit to the program as a group will be a standardized format for analysis of specimens from the common animal models that will be using different experimental approaches/targets. This will provide valuable information that can be shared and compared across the projects in the program. To accomplish these goals, the core is directed by Dr. Kenneth Kozloff, who is an expert in bone biology. In addition, the core is supported by Dr. Rohit Mehra, a board-certified urologic oncology pathologist and Dr. Todd Morgan, a urologist and physician scientist with expertise in disseminated tumor cell procurement and biology. Additional expertise in bone histomorphometry is provided by our consultant Dr. Tom Wronski a nationally recognized expert in this field and in bone pathology by Dr. David Lucas, an anatomic pathologist who specializes in bone. Overall this core will continue to provide comprehensive and unique services that are required by all the projects of this Program.