Core C: Metabolic Phenotyping Core

摘要/摘要 目前计划的目标是加深我们对细胞内分子机制的理解 有助于心脏代谢控制的大脑。当前的核心旨在提供技术和 对所有四个项目所采用的小鼠模型能量动态平衡评估的智力支持 在这个项目中。通过这一核心，项目将能够使用一系列设备来评估 能量动态平衡，包括多路系统(扑草净、OxyMax)、基于核磁共振的身体成分 分析仪，弹式量热计，代谢笼系统，呼吸仪，直接量热计，细胞 呼吸系统、尿液分析/血液分析系统等。此外，核心工作人员将提供手术支持 对于所有项目，PI将为所有项目提供统计支持和培训。将这些集中在一起 进入核心的端点将支持提高所有代谢表型数据的严密性和可重复性 这个项目。

Summary / Abstract The objective of the current program is to further our understanding of the molecular mechanisms within the brain that contribute to cardiometabolic control. The current core is designed to provide technical and intellectual support for assessments of energy homeostasis in mouse models employed by all four projects within the program. Through this core, projects will have access to an array of equipment used to assess energy homeostasis including multiplexed systems (Promethion, OxyMax), NMR-based body composition analyzers, bomb calorimeters, metabolic caging systems, respirometers, direct calorimeters, cellular respiration systems, urinalysis/blood analysis systems, etc. In addition, core staff will provide surgical support for all projects, and the PI will provide statistical support and training for all projects. The centralizing of these endpoints into a core will support increased rigor and reproducibility of all metabolic phenotyping data across the program.