DNA Core

DNA合成资源核心 DNA合成资源核心在本计划项目的成功整合中起着至关重要的作用 通过向项目1、2和3提供高纯度、位点特异性修饰的寡核苷酸。DNA合成 Resource Core与项目1密切合作，开发新的试剂和合成策略， 位点特异性合成含有该计划感兴趣的Fapy-dG病变的寡核苷酸 项目将该技术转移到核心，在那里将所需的修饰的寡核苷酸 以适当的规模和纯度合成，用于进一步的化学品（项目1）和生物化学品（项目1 和2）研究，基于细胞的研究（项目2），以及结构和构象分析（项目3）。的 DNA合成资源核心的具体目标是：1）合成位点特异性修饰的 2）开发用于纯化修饰的寡核苷酸的方案; 寡核苷酸;和3）提供修饰的寡核苷酸的初始表征。

DNA Synthesis Resource Core The DNA Synthesis Resource Core plays an essential role in the successful integration of this Program Project by providing high purity, site-specifically modified oligonucleotides to Projects 1, 2 and 3. The DNA Synthesis Resource Core works closely with Project 1, where novel reagents and synthetic strategies are developed for the site-specific synthesis of oligonucleotides containing the Fapy-dG lesions of interest to the Program Project. The technology is transferred to the Core, where the desired modified oligonucleotides are synthesized on the appropriate scale and purity for further chemical (Project 1) and biochemical (Projects 1 and 2) studies, cell-based studies (Project 2), and structural and conformational analyses (Project 3). The Specific Aims for the DNA Synthesis Resource Core are to: 1) synthesize site-specifically modified oligonucleotides of interest to this PPG; 2) develop protocols for the purification of the modified oligonucleotides; and 3) provide initial characterization of modified oligonucleotide.