Resource Section

项目摘要资源核心 体细胞基因组编辑(SCGE)联盟的总体目标是加速发展 基因组编辑技术的翻译应用，特别是CRISPR/Cas9，利用最近的 基因治疗的成功。杰克逊实验室基因组编辑小鼠的具体目标 测试中心(JAX-GEMTC)是为了开发新的鼠标资源，以便于验证和优化 新的基因组编辑技术。JAX-GEMTC的资源核心将支持老鼠育种 和菌落扩张，用于记者的基因和功能验证、队列生产和整合 与NIH资助的小鼠突变区域资源中心(MMRRC‘s)合作。资源将会利用 JAX KOMP2程序的高通量菌落管理基础设施和鼠标资源 JAX存储库的分发经验。这些新资源的公开可获得性将通过 中心与日本宇宙航空研究开发机构MMRRC的共同本地化。中心的资源目标将会实现 通过两个具体目标：1)建立报道线生产的规模化繁育群体 JAX-GEMTC的队列。这个目标的目标是建立报告鼠的生产群体，以支持所有 JAX-GEMTC计划的各个方面。初步来说，我们会引入现有的交通灯报告器模式，以 贾克斯随后还将额外引进记者。JAX作为动物的一部分设计的模型 将对模型生产区进行生殖系传播筛查，并对所有模型的生产菌落进行 建立功能验证、基线测试，并为测试核心提供队列。2) 通过日本宇宙航空研究开发机构MMRRC将记者专线存档并分发给科学界。为了实现这一目标，我们将 提供新颖的报告线路和所有相关信息，以供MMRRC使用，以便分发给 科学界。为了实现这一目标，我们将利用JAX作为MMRRC中心之一的角色， 促进提交、存档和分发过程。

PROJECT SUMMARY RESOURCE CORE The overall goal of the Somatic Cell Genome Editing (SCGE) consortium is to accelerate the development of translational applications of genome-editing technology, particularly CRISPR/Cas9, taking advantage of recent successes in gene therapy treatments. The specific goal of the Jackson Laboratory Genome Editing Mouse Testing Center (JAX-GEMTC) is to develop novel mouse resources to facilitate the validation and optimization of new genome editing technologies. The RESOURCE CORE of the JAX-GEMTC will support mouse breeding and colony expansion for genotypic and functional validation of reporters, cohort production, and integration with the NIH-funded Mouse Mutant Regional Resource Centers (MMRRC’s). The resource will take advantage of the high-throughput colony management infrastructure of the JAX KOMP2 program and the mouse resource distribution experience of the JAX Repository. Public availability of these new resources will be facilitated by the co-localization of the Center with the JAX MMRRC. The Resource goals of this Center will be achieved through two Specific Aims: 1) Establish production-scale breeding colonies for production of reporter line cohorts for the JAX-GEMTC. The goal of this Aim is to build production colonies of reporter mice to support all aspects of the JAX-GEMTC program. Initially, we will import the existing Traffic Light Reporter (TLR) model to JAX and then the importation additional reporters will follow. Models engineered at JAX as part of the Animal Model Production section will be screened for germline transmission, and production colonies of all models will be established for functional validation, baseline testing, and to provide cohorts for the TESTING CORE. 2) Archive and distribute reporter lines to the scientific community through the JAX-MMRRC. In this aim we will provide novel reporter lines and all associated information for their use to the MMRRC for distribution to the scientific community. To accomplish this we will take advantage of JAX’s role as one of the MMRRC centers, facilitating the submission, archiving, and distribution process.