Studying Dynamics of Monocyte HIV RNA Persistence and the Interface with CNS Outcomes during Acute HIV Infection

我的工作目标是阐明在髓系细胞中潜伏的艾滋病毒感染作为一种 更好地了解髓系免疫功能障碍、炎症和早期 在急性艾滋病毒感染(AHI)期间认知能力下降。我们的初步数据显示单核细胞 相关的HIV DNA数据与单核细胞携带HIV DNA的假设是一致的 以及早期感染过程中的RNA。扩展我们正在进行的单核细胞评估研究 相关的HIV DNA，我打算事先调查血液和脑脊液中单核细胞HIV RNA负荷 在辅助抗炎治疗的背景下进行ART干预和术后干预 啊嗨。RV408/SEARCH018队列提供了引导我建议的单个电池的途径 单核细胞表型和单细胞RNA测序的评估。我的目标带来的结果 将成为定义早期HIV RNA转录活性的基础，目前我们的 了解单核细胞相关的HIV RNA负荷。

The goal of my work is to elucidate the role of latent HIV infection in myeloid cells as an approach to better understand myeloid immune dysfunction, inflammation and early stages of cognitive decline during acute HIV infection (AHI). Our preliminary data show monocyte associated HIV DNA data are consistent with the hypothesis that monocytes harbor HIV DNA and RNA during early infection. Expanding on our on-going studies evaluating monocyte associated HIV DNA, I intend to investigate monocyte HIV RNA burden in blood and CSF prior to and post-ART intervention and in the context of adjunctive anti-inflammatory therapy during AHI. The RV408/SEARCH018 cohort provides access to conducting my proposed single cell assessments for monocyte phenotype and single cell RNA sequencing. Results from my aims will form the basis for defining early HIV RNA transcriptional activity to our presently limited understanding of monocyte associated HIV RNA burden.