Geroscience Redox Biology Core

Geroscience Redox生物学核心的目标是为研究人员提供一个协调和 全面评价有助于氧化代谢的分子、途径和系统， 氧化应激这些分析包括最先进的氧化还原和能量的准确和灵敏的措施 平衡以及氧化损伤、活性氧产生和线粒体功能。氧化还原 状态和氧化剂体内平衡对生理过程具有主要影响。因此，他们 对衰老的基本机制以及一些与年龄有关的疾病有重大贡献， 老年科学中的路径本核心的具体目标如下：具体目标1。执行准确 氧化还原状态（GSH/GSSG、NADPH/NADP+、NADH/NAD+）和氧化应激的综合分析 和损伤标志物（脂质过氧化，例如，F2-异前列烷;蛋白质氧化，例如，羰基;和DNA 氧化，例如8-氧代-脱氧鸟苷）。氧化还原和氧化损伤终点的存在量有限 并且可能不稳定。Geroscience Redox Biology Core的专业知识使这些分析能够 以尽可能高的灵敏度和准确度进行。此外，还分析了老年科学的氧化还原 生物学核心课程特别适合研究核心课程，因为它们需要昂贵的设备 和重要的技术专长，防止这些类型的分析，以例行措施，在个人 laboratories.最后，由于我们分析的是高度特异性的生物化合物水平，我们的氧化还原和 氧化损伤测定可应用于来自任何活生物体的组织或细胞。具体目标2。提供 线粒体功能、能量状态和活性氧产生的测定 组织、分离的线粒体和细胞培养模型。虽然这些检测需要新鲜的组织/细胞，但我们有 成功地为外部研究中心的研究者提供了这些服务，这些研究中心可以向我们发送少量小鼠。 具体目标3。为了提供自由基产生的体内分析，我们扩大了 Geroscience氧化还原生物学核心在这次更新中，包括氧化还原和能量状态，血管 功能障碍和组织结构变化。Geroscience氧化还原生物学核心现在将提供体内氧化还原 使用磁共振成像（MRI）和免疫自旋共振（MRI）分析自由基水平和氧状态 捕获（IST）。此外，功能性MRI（fMRI）将用于从以下患者获得的氧状态和能量状态： 组织定位的磷（31 P）-和氢（1H）-MR光谱。 总的来说，Geroscience Redox生物学核心将提供一个全面的终点标志物小组， 氧化还原平衡和氧化应激以及影响因素的测量，如线粒体功能 以及体内和体外自由基的产生，这会影响到衰老和老年科学的许多方面。

The goal of the Geroscience Redox Biology Core is to provide investigators with a coordinated and comprehensive evaluation of the molecules, pathways, and systems that contribute to oxidative metabolism and oxidative stress. These analyses include state of the art accurate and sensitive measures of redox and energy balance as well as oxidative damage, reactive oxygen species production and mitochondrial function. Redox status and oxidant homeostasis have a major impact on physiologic processes. As a consequence, they contribute significantly to basic mechanisms of aging as well as a number of age-related diseases, and are key pathways in geroscience. The Specific Aims for this Core are the following: Specific Aim 1. To perform accurate and comprehensive analyses of redox status (GSH/GSSG, NADPH/NADP+, NADH/NAD+,), and oxidative stress and damage markers (lipid peroxidation, e.g., F2-isoprostanes; protein oxidation, e.g., carbonyls; and DNA oxidation, e.g. 8-oxo-deoxyguanosine). Redox and oxidative damage endpoints are present in limited amounts and may be unstable. The expertise in the Geroscience Redox Biology Core allows for these analyses to be performed with the highest possible sensitivity and accuracy. In addition, the analyses the Geroscience Redox Biology Core provides are especially appropriate for a Research Core because they require costly equipment and significant technologic expertise that prevents these types of analyses to routine measures in individual laboratories. Finally, because we are analyzing levels of highly specific biological compounds, our redox and oxidative damage assays can be applied to tissues or cells from any living organism. Specific Aim 2. To provide assays of mitochondrial function, energetic status, and reactive oxygen species generation using permeabilized tissue, isolated mitochondria, and cell culture models. Although these assays require fresh tissue/cells, we have successfully performed these services for investigators at external sites who can send us small numbers of mice. Specific Aim 3. To provide in vivo analyses of free radical production, we have expanded the capabilities of the Geroscience Redox Biology Core in this renewal to include in vivo analyses of redox and energy status, vascular dysfunction, and tissue structural changes. The Geroscience Redox Biology Core will now provide in vivo redox analyses of free radical levels and oxygen status using magnetic resonance imaging (MRI) and immuno-spin trapping (IST). In addition functional MRI (fMRI), will be used for oxygen status and energy status obtained from tissue-localized phosphorous (31P)- and hydrogen (1H)-MR spectroscopy. Overall, the Geroscience Redox Biology Core will provide a comprehensive panel of endpoint markers of redox balance and oxidative stress as well as measures of contributing factors such as mitochondrial function and free radical generation in vitro and in vivo that can impact many aspects of anging and Geroscience.