THE IMMUNE SELF-ASSOCIATED STORAGE ORGANELLE (SASO)

项目总结 这项研究调查了身体如何保持对“自我”的记忆。当面对外来生物和癌症时， 这种自我认识被认为是至关重要的，有助于教育免疫系统限制自身免疫和 相反，许可对“外来”事物的反应性。这项研究的核心是一种新发现的 巨噬细胞和一些树突状细胞内的细胞器，我们称之为自相关存储 细胞器或简称为Saso。我们相信Saso对于‘保持’我们的自我认同以及随后的 教育我们免疫系统的其余部分，它似乎来自一种隐形的微妙形式的细胞 我们的方案在应用切割技术方面是独一无二的-晶格光片成像、泡泡流、质量- 光谱和电子显微镜--结合常规细胞免疫分析方法和遗传学 了解这种新描述的免疫系统组件是如何工作的，以及我们是如何 可以利用它来改善健康。由此产生的发现将对设计新的促进 抗肿瘤免疫，以最大限度地减少自身免疫，并广泛地调节我们身体的哪些组成部分 以“自我”的身份。

PROJECT SUMMARY This study investigates how the body maintains a memory of ‘Self’. When facing foreign organisms and cancer, this self-knowledge is thought to be critical, serving to educate the immune system to limit autoimmunity and conversely license reactivity to things that are ‘Foreign’. The core of the study focuses on a newly-discovered organelle, found within macrophages and some dendritic cells which we call the Self-Associated Storage Organelle or SASO for short. We believe that the SASO is critical to ‘hold’ our self-identity and subsequently educate the rest of our immune system and it appears to come from a stealthy and subtle form of ‘cell nibbling’.Our proposal is unique in applying cutting technologies—lattice-light sheet imaging, vesicle flow, Mass- spectrometry and Electron Microscopy—together with conventional cellular immunoassay methods and genetic screens, to understand how this newly described component of the immune system works as well as how we can harness it to improve health. The resultant discoveries will be formative for designing new ways to boost anti-tumor immunity, to minimize autoimmunity and to broadly regulate which components of our bodies are seen as ‘Self’.