Molecular epidemiology of respiratory virus exposure in elementary schools

项目总结/摘要 在理解学校环境在多大程度上有助于 呼吸道病毒暴露，以及对减少暴露的潜在环境干预知之甚少。 我们的长期目标是为儿童创造健康的室内学校环境。本报告的总体目标 应用是确定小学呼吸道病毒暴露的可改变因素， 降低哮喘发病率。我们将结合联合收割机的新方法来量化和表征呼吸系统。 通过利用学校内城哮喘干预研究的强大基础设施， （SICAS-2，ClinicalTrials.gov NCT 02291302），一项随机对照试验，由我们的研究小组进行， 安慰剂对照教室高效微粒空气（HEPA）清洁剂干预。我们将测试我们的 中心假设：学校是影响哮喘发病率的呼吸道病毒高暴露源 （1）比较学校与非学校接触呼吸道病毒的情况， 通过使用混合捕获面板恢复检测到的呼吸道病毒的基因组， 预测学校呼吸道病毒暴露的因素，包括便携式HEPA清洁器在 减少教室呼吸道病毒暴露;（3）确定教室 呼吸道病毒与小学生哮喘症状这种方法是创新的，因为 我们正在同时调查学校环境中所有主要的人类呼吸道病毒。的 拟议的研究是重要的，因为它将确定暴露于呼吸道病毒的程度， 学校和潜在的环境因素，可以作为未来的干预目标。如果HEPA 干预可以减少呼吸道病毒暴露，这是一种立即可采取行动和实际的干预措施， 为小学生创造更安全的室内环境。该提案的结果可能是 可推广到其他公共室内环境。

PROJECT SUMMARY/ABSTRACT There is a fundamental gap in understanding the extent to which the school environment contributes to respiratory virus exposure, and less is known about potential environmental interventions to reduce exposure. Our long-term goal is to create healthy indoor school environments for children. The overall objective of this application is to identify modifiable factors of respiratory virus exposure in elementary schools in order to reduce asthma morbidity. We will combine novel approaches to quantify and characterize the respiratory virome in schools by leveraging the robust infrastructure of the School Inner-City Asthma Intervention Study (SICAS-2, ClinicalTrials.gov NCT02291302), a randomized controlled trial conducted by our group of a placebo-controlled classroom high efficiency particulate air (HEPA) cleaner intervention. We will test our central hypothesis that schools are a source of high respiratory virus exposure that affects asthma morbidity through the following specific aims: (1) To compare respiratory virus exposure in schools as compared to homes by using a hybrid capture panel to recover genomes of detected respiratory viruses; (2) To identify factors predicting respiratory virus exposure in schools including the efficacy of portable HEPA cleaners in reducing classroom respiratory virus exposure; (3) To determine the association between the classroom respiratory virome and asthma symptoms in elementary school children. The approach is innovative, because we are simultaneously interrogating all major human respiratory viruses in the school environment. The proposed research is significant, because it will identify the magnitude of exposure to respiratory viruses in schools and potential environmental factors that can serve as future targets of intervention. If the HEPA intervention can reduce respiratory virus exposure, it is an immediately actionable and practical intervention to create safer indoor environments for elementary school children. Results from this proposal may be generalizable to other public indoor settings.