Circadian regulation of neocortex

项目摘要 人们对决定休息活动每日节律的机制知之甚少。我们最近 研究发现，运动皮质内的细胞可以与糖皮质激素的日常周期同步。 我们假设每日视交叉上核活动和皮质酮分泌 大脑皮层神经元和星形胶质细胞的昼夜节律来调节日常行为模式。我们 将利用新的高通量、机器学习以及记录和 操纵小鼠特定细胞类型的基因表达和钙水平。将这些组合在一起 方法用双色实时成像技术同时检测神经元和 对于星形胶质细胞，我们将确定皮质神经元和胶质细胞在不同日常活动中的作用。

Project Summary Little is known about the mechanisms that determine daily rhythms in rest-activity. We recently found that cells within the motor cortex can be synchronized to daily cycles of glucocorticoids. We hypothesize that daily suprachiasmatic nucleus activity and corticosterone secretion entrain circadian rhythms in cortical neurons and astrocytes to regulate daily patterns of behavior. We will take advantage of new high-throughput, machine learning and the ability to record and manipulate gene expression and calcium levels in specific cell types in mice. Combining these methods with two-color, real-time imaging of gene expression simultaneously in neurons and astrocytes, we will determine the roles of cortical neurons and glial in distinct daily activities.