Demonstrate the molecular receptor and functions of dorsal organ warm cells in flies

摘要-Lina Ni 动物依靠它们的温度传感系统来避免有害的极端温度，并寻找 生存的最佳温度。体温感觉与小动物特别相关，例如 昆虫，它们依靠环境温度来设定它们的体温。许多传播疾病的昆虫， 例如蚊子，对它们温血宿主的温度做出反应，并用它来引导血液- 它们通过觅食行为传播人类疾病。因此，重要的是要确定温度- 感应分子和神经元，以帮助控制疾病媒介。果蝇是一个适合这些系统的模型系统 研究，因为他们进化保守的温度感应分子与蚊子。在苍蝇身上，有许多 温度传感系统拥有少量掌握强健的温度感觉神经元 行为。此外，在苍蝇身上开发的强大的遗传学确保了对温度的精确控制- 感知分子和神经元。私家侦探实验室的初步研究发现了一组以前未确认的 蝇幼虫的热激活神经元，其感温分子和功能尚不清楚。 初步数据表明，这些神经元依赖于一类新的温感分子来检测 温暖的温度。除了温度反应神经元的经典功能外，它还决定了温度 偏爱，这些神经元可能有一个额外的功能，以调节其邻近的冷激活神经元。 这种调节可能会导致邻近神经元的生理特性发生变化。这样做的目的是 应用是识别这些热激活神经元中的温敏分子，以证明它们的 在温度偏好中的作用，并确定它们对邻近神经元的调制作用。在……里面 除了有初步数据外，我们特别准备好进行拟议的研究 由于我们在研究温度响应性分子和 苍蝇的感觉神经元。由于温敏分子的潜在候选者之间是保守的 苍蝇和蚊子，这项研究可能为控制蚊子的觅血行为提供新的靶点 和其他疾病传播者。

Summary – Lina Ni Animals depend on their temperature-sensing systems to avoid noxious thermal extremes and to seek optimal temperatures for survival. Temperature sensation is particularly relevant for small animals, such as insects, which rely on ambient temperatures to set their body temperatures. Many insect vectors of diseases, such as mosquitoes, respond to the temperature of their warm-blooded hosts and use it to guide the blood- feeding behaviors through which they transmit human diseases. Thus, it is important to identify the temperature- sensing molecules and neurons to help control disease vectors. Fruit flies are a suitable model system for these studies, because of their evolutionarily conserved temperature-sensing molecules with mosquito. In flies, many temperature-sensing systems possess a small number of temperature sensory neurons that master robust behaviors. Moreover, the powerful genetics developed in flies ensures the precise manipulation of temperature- sensing molecules and neurons. Preliminary studies from the PI’s lab discovered a set of previously unidentified warm-activated neurons in fly larvae, whose temperature-sensing molecules and functions are unknown. Preliminary data suggested that these neurons depended on a new class of warm-sensing molecules to detect warm temperatures. Besides a classic function of temperature-responsive neurons that determine temperature preference, these neurons may have an additional function to modulate their neighboring cool-activated neurons. Such modulation may result in change of physiological properties in neighboring neurons. The goal of this application is to identify the warm-sensing molecules in these warm-activated neurons, to demonstrate their functions in temperature preference, and to determine their modulatory effects on neighboring neurons. In addition to having preliminary data, we are particularly well-prepared to undertake the proposed research because of our extensive, and successful, track record of studying temperature-responsive molecules and sensory neurons in flies. Since the potential candidates of the warm-sensing molecules are conserved between flies and mosquitoes, this study might provide novel targets to control blood-feeding behaviors of mosquitoes and other disease vectors.