Versatile femtosecond technology for adaptive multi-photon imaging

摘要 这项研究计划的目标是开发飞秒源技术，超越模式， 锁定激光器，并展示这些新技术与多光子显微镜的基础上，自适应 激发基于自适应激励的高速多光子成像需要波长和脉冲 模式灵活性，目前还没有。此外，传统的基于激光的源在以下方面受到限制： 波长、重复率、复杂度和成本。这项研究将首先证明所需的 多用途飞秒光源，并将其应用于基于自适应 激发该研究计划基于三大创新：（1）高质量飞秒脉冲 通过光调制和新颖的非线性脉冲压缩和净化， 技术，（2）高能量波长捷变放大可以通过宽带在光纤中实现 啁啾脉冲的脉冲内相位匹配，以及（3）自适应激励使得能够实现比脉冲的阶数更多的相位匹配。 大幅度提高了多光子显微镜的速度， 任意波长和脉冲图案的组合，例如与所提出的鲁棒光纤格式 技术创新（1）和（2）。我们的目标是使更多的数量级 在极限深度处对帧速率的改进限制了使用新源的成像， 比以前的技术更容易使用。该计划的成功完成将产生重大影响 用于采用超短脉冲技术的生物医学研究方向，例如深部组织成像。

Abstract The goal of this research program is to develop femtosecond source technologies that move beyond mode- locked lasers and to demonstrate these new techniques with multiphoton microscopy based on adaptive excitation. High speed multiphoton imaging based on adaptive excitation requires wavelength and pulse pattern agility that is not currently available. Traditional laser-based sources, moreover, are limited in wavelength, repetition rate, complexity, and cost. The research proposed will first demonstrate the desired versatile femtosecond source and then apply this source to multiphoton microscopy based on adaptive excitation. The research program is based on three major innovations: (1) high quality femtosecond pulse trains can be generated through optical modulation and novel nonlinear pulse compression and cleaning techniques, (2) high energy wavelength-agile amplification can be achieved in fiber through broadband intra-pulse phase matching of chirped pulses, and (3) adaptive excitation enables more than order-of- magnitude improvements to the speed of multiphoton microscopy with a high power source supporting the combination of an arbitrary wavelength and pulse pattern, such as with the proposed robust fiber-format technique enabled by innovations (1) and (2). Our aim is to enable more than an order-of-magnitude improvement to frame rates at the ultimate depth limits to imaging with a novel source that is significantly more accessible than previous technologies. Successful completion of this program will have major impact for biomedical research directions which employ ultrashort pulse technology, such as deep tissue imaging.