Diversity Research Supplement for Combining Experiments and Computer Simulation to Improve the Stem Cell Differentiation Process

摘要 这项工作的总体目标是确定微米级和纳米级PAR的运动特性。 flUID中的TILES，这对于研究干细胞中细胞内内容物的运动具有直接的应用 父母拨款中提出的差异化建议。需要对flUID属性进行清晰的评估，因为它具有 这对计算机模拟干细胞分化的准确性有很大影响。一种光学镊子装置 将被用来观察微米和纳米大小的珠子在接近镊子焦点时的运动 点以确定它们的运动是否过度、不足或甚至严重受阻。flUID VIS- COSITY也将发生变化，以复制细胞质和核质的COSITY。我们的想法是描述这种转变的特征 在过阻尼力和欠阻尼力运动之间，以及发生这种转变的条件(如果发生的话)。 这一评估很重要，因为建议的单元格模型包括上下大小的对象 那就是应该发生转变的地方。这项研究的结果，连同父母拨款中建议的结果， 应该有助于开发准确的干细胞分化的高速模型，以帮助 fi填补了目前对细胞机械生物学了解的空白，并促进了未来对其他细胞机械生物学的研究 机械生物学过程，如伤口愈合。这项研究还应该导致一项联合实验 以及探索细胞机械生物学的理论光学镊子工具。

ABSTRACT The overall goal of this work is to determine the characteristics of the motion of micro-sized and nano-sized par- ticles in a fluid, which has direct application to the study of the motion of intracellular contents during stem cell differentiation proposed in the parent grant. A clear assessment of the fluid properties is needed because it has a great impact on the accuracy of the computer simulation of stem cell differentiation. An optical tweezer apparatus will be used to observe the motion of micron-sized and nano-sized beads as they approach the tweezer's focal point to determine whether their motion is overdamped, underdamped or even critically damped. The fluid vis- cosity will also be varied to replicate that of cytoplasm and nucleoplasm. The idea is to characterize the transition between overdamped and underdamped motion and the conditions under which this transition occurs, if it occurs. This assessment is important because the proposed model of the cell includes objects of a size above and below that where the transition should occur. The result of this study, along with those proposed in the parent grant, should facilitate the development of an accurate high-speed model of stem cell differentiation that can assist in filling gaps in the current understanding of cellular mechanobiology, and facilitate future studies of other cellular mechanobiological processes such as wound healing. This study should also result in a combined experimental and theoretical optical tweezer tool for exploring the mechanobiology of cells.