PacBio Sequel IIe for GCB Sequencing Core

杜克测序和基因组学技术共享资源（SGT）为生物医学领域的PI提供服务 从杜克医学院到整个杜克。SGT是一个约6600平方英尺的最先进的设施， 拥有超过310万美元的仪器设备，并拥有10名全职员工。十多年来， SGT始终提供最新的、最先进的基因组服务。与大多数实验室一样， DNA/RNA测序是SGT中测序技术的主要形式，因为它高度准确 并且成本效益高。然而，短读测序无法访问复杂的基因组区域。以补充 除了短读段测序方法之外，包括SGT在内的实验室使用长读段测序技术。因此， SGT为PI提供高度准确的短Illumina测序读数和中等准确的超长测序读数。 Oxford Nanopore测序读数。然而，SGT目前不提供高度准确的长 由PacBio Sequel IIe系统产生的测序读数。续集IIe，这本书中要求的工具 该提案，将是一个独特的资源，杜克大学和学术机构在北卡罗来纳州。PacBio 最近克服了长期困扰长读技术的弱点。这些弱点是 不准确、低生产量和高成本。第一个由PacBio Sequel IIe测序的HiFi文库 是非常准确的。接下来，研究人员和PacBio开发的多种技术提高了吞吐量 长读段测序此外，Sequel IIe具有本地计算能力，可以减少劳动力。采取 PacBio Sequel IIe为PI提供8倍的数据量，总体成本降低50%。 获得Sequel IIe将允许PI以成本效益高的方式询问病毒的重复区域， 区分基因组区域以鉴定与疾病相关的重复序列，直接检测重组 事件和删除，剪接和定相，等等。鉴于续集的要求，我将是唯一的 仪器迎合了NC学术研究人员的需求，该仪器不仅有利于NIH- 资助的PI在杜克医学院，但在所有的杜克和北卡罗来纳州。

The Duke Sequencing and Genomics Technology Shared Resource (SGT) serves PIs in the biomedical fields from Duke’s School of Medicine and all over Duke. The SGT is a ~6600 sq foot state-of-the-art facility that houses more than $3.1M of instrumentation and has ten full time employees. For more than a decade, the SGT consistently has provided updated, state-of-the-art genomic services. Like most laboratories, short-read DNA/RNA sequencing is the dominant form of sequencing technology in the SGT because it is highly accurate and cost-effective. However, short-read sequencing cannot access complex genomics regions. To complement short-read sequencing methods, labs including the SGT use long-read sequencing technologies. As such, the SGT provides PIs with highly accurate short Illumina sequencing reads and moderately accurate ultra-long Oxford Nanopore sequencing reads. However, the SGT currently does not offer highly accurate long sequencing reads produced by the PacBio Sequel IIe system. The Sequel IIe, the instrument requested in this proposal, would be a unique resource to Duke University and academic institutes in North Carolina. PacBio has recently overcome weaknesses that have long plagued long-read technology. These weaknesses are inaccuracy, low-throughput production, and high cost. First HiFi libraries sequenced by the PacBio Sequel IIe are highly accurate. Next multiple technologies developed by researchers and PacBio increase the throughput of long-read sequencing. Additionally, the Sequel IIe has local computational abilities that reduces labor. Taken together, the PacBio Sequel IIe provides PIs with 8x the amount of data at an overall 50% cost reduction. Acquisition of a Sequel IIe would allow PIs to cost-effectively interrogate repetitive regions of viruses, size discrimination of genomic regions to identify repeats correlated to disease, direct detection of recombination events and deletion, splicing and phasing, and more. Given the requested Sequel IIe would be the only instrument catering to the needs of academic researchers in NC, this instrument would not only benefit NIH- funded PIs in Duke’s School of Medicine, but across all of Duke and North Carolina.