Light-Seq: Spatially targeted profiling of transcriptomic states in cells and tissue

总结 我们将开发一个新的空间组学平台Light-Seq，用于完整生物样品的空间索引， 在固定的细胞和组织中进行光引导的DNA条形码化，然后进行非原位测序。我们的光导向 条形码策略将使用户能够指导，原位选择罕见的，不相交的细胞群， 基于形态、位置或蛋白质表达的转录组测序而不解离。我们将 开发Light-Seq作为能够提取转录组信息的空间组学DNA条形码平台 从单细胞，可扩展到唯一地解决数千个用户定义的区域，并可以应用于 固定和FFPE临床样品，用于直接应用于人类健康。我们设想Light-Seq平台 将是一个可扩展的，具有成本效益的，灵活的空间转录组学方法，允许用户定义 用于NGS测序的组织中的空间区域。因此，Light-seq可以作为一个低门槛的进入平台， 空间转录组学为许多病理学家和研究人员，并将成为一个关键的驱动因素，更广泛地采用 空间转录组学工具。

Summary We will develop a new spatial-omics platform, Light-Seq, for spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Our light-directed barcoding strategy will enable user-directed, in situ selection of rare, disjoint cell populations for full- transcriptome sequencing based on morphology, location, or protein expression without dissociation. We will develop Light-Seq as a spatial-omic DNA barcoding platform capable of extracting the transcriptomic information from single-cells, scalable to uniquely address thousands of user-defined regions, and can be applied in both fixed and FFPE clinical samples for direct applications in human health. We envision that the Light-Seq platform will be a scalable, cost-effective, and flexible approach to spatial transcriptomics that allows the user to define spatial regions in tissue for NGS sequencing. Light-seq can thus serve as a low barrier-to-entry platform for spatial transcriptomics for many pathologists and researchers, and would be a key driver for a wider adoption of spatial transcriptomic tools.