Host Microbe Core

摘要 宿主微生物核心(HMC)将协助和支持研究基地、受训人员和试点项目 调查人员通过提供标本、试剂、培训和服务来确定微生物和宿主 NIDDK优先考虑器官和组织中的CF病的反应决定因素。此核心结合了 CF基础和临床微生物学、呼吸道上皮和免疫细胞专家的资源和专业知识 生物学、病理学和显微镜提供综合服务网络，反映了 对CF病发病机制领域进行同时调查寄主和病原菌。提供核心服务 UW CF P30研究基地包括： 1.为组织病理学、免疫组织化学、原位研究提供技术援助和最先进的设施 宿主和微生物靶标的杂交和定位，以及数字成像 2.人胰腺和肺的定量数字病理和全幻灯片数字信息库 使用CF 3.处理临床、动物和实验室样本中的微生物组、蛋白质组、代谢组和 转录组分析，以及针对特定靶点 4.以代表用于表型的CF粘膜条件的形式对CF样品进行微生物培养， 蛋白质组、基因组和生化分析 5.接触从CF临床粪便样本培养的细菌菌株，包括大肠杆菌和 拟杆菌属 6.核心提供的方法和实验设计方面的咨询和培训 7.与临床和基因组学核心密切互动，为CF研究人员提供统一服务

ABSTRACT The Host Microbe Core (HMC) will assist and support the research base, trainees, and pilot project investigators by providing specimens, reagents, training, and services to define both the microbial and host response determinants of CF diseases in organs and tissues prioritized by the NIDDK. This Core combines the resources and expertise of specialists in CF basic and clinical microbiology, airway epithelial and immune cell biology, pathology, and microscopy to provide an integrated network of services, reflecting an evolution in the field of CF disease pathogenesis to concurrently investigate the host and pathogens. Core services available to the UW CF P30 research base include: 1. Technical assistance and state-of-the-art facilities for histopathology, immunohistochemistry, in situ hybridization and localization of host and microbial targets, and digital imaging 2. Quantitative digital pathology and whole slide digital repository for pancreata and lungs from people with CF 3. Processing clinical, animal, and laboratory specimens for microbiome, proteome, metabolome, and transcriptome analyses, and for specific targets 4. Microbial culturing of CF specimens in formats representative of CF mucosal conditions for phenotypic, proteomic, genomic, and biochemical analyses 5. Access to strains of bacteria cultured from CF clinical fecal samples, including Escherichia coli and Bacteroides spp. 6. Consultation and training in methods provided by the Core and experimental design 7. Interacting closely with the Clinical and Genomics cores to provide unified services for CF researchers