Genomics and Cell Biology Core

项目摘要/摘要 基因组学与细胞生物学核心的总体目标将是促进尖端技术的应用 基因组学、生物信息学、细胞生物学和免疫学技术应用于营养和代谢研究。这 将通过鼓励多学科方法和合作来研究营养相关问题来实现 主题，并克服使用复杂和昂贵技术的障碍，通过提供适当的 向NORCH调查员提供咨询和培训。基因组学的指导思想&细胞生物学 核心是尽管许多项目可以通过使用复杂的基因组和细胞生物学来推进 技术、费用和首次使用的困难是一个很大的障碍。核心是明确的 旨在通过为NORC-H调查人员提供个性化咨询和培训来克服这一障碍 这在大多数NGS、基因组学、生物信息学、细胞生物学或免疫学核心设施中通常是不可用的。 在核心主任弗雷德·奥苏贝尔博士退休后，他一直担任基因组学核心主任 该中心自1994年以来最初的赠款)，Alessio Fasano博士，一种著名的腹部疾病 研究员和之前的共同核心董事，已经承担了董事的角色。联席董事 (生物信息学和下一代测序)Ruslan Sadreyev博士和联席副主任(细胞 生物学)施海宁博士将继续负责核心的各个部分。该计划有5个具体目标 核心：1)提供与基因组学、细胞生物学、免疫学研究设计相关的咨询服务；2) 为专业技术的使用提供空间、技术人员和专门的培训和监督 以及用于细胞生物学、免疫学和基因组学研究的设备，包括单细胞转录 和表观基因组图谱，帮助开发高含量、高通量的分析方法 自动化显微成像、荧光激活细胞分选和动物模型的建立；3) 提供与下一代测序相关的各种基因组学相关服务；4)提供生物信息学 分析各种类型的高通量数据，包括散装和单细胞RNA-SEQ、ATAC-SEQ、CHIP-SEQ SEQ，Cut&Run，Hi-C，元基因组学，蛋白质组学，以及分子途径的计算分析， 网络和表型；以及5)举办关于基因组基本概念的丰富教程和讲习班， 生物信息学、细胞生物学、营养学和胃肠道免疫学相关技术，并协调夏季 本科生和医学生的实习机会。这将通过重要的 核心主任和工作人员的专门知识，借助先进的生物信息学分析仪器， 最新的新一代测序仪器、自动显微镜和基于高 内容筛选分析，用于进行免疫学、细胞生物学和形态学的各种设备- 相关检测。

PROJECT SUMMARY/ABSTRACT The overall goal of the Genomics & Cell Biology Core will be to facilitate the application of cutting-edge genomics, bioinformatics, cell biology, and immunology techniques to nutrition and metabolic research. This will be accomplished by encouraging multi-disciplinary approaches and collaborations to study nutrition-related topics, and by overcoming the barriers to using complex and expensive techniques by providing appropriate consultation and training to NORCH Investigators. The guiding philosophy of the Genomics & Cell Biology Core is that although many projects can be advanced by the use of sophisticated genomic and cell biology techniques, their expense and difficulty of first use represent a significant barrier. The Core is specifically designed to overcome this barrier by providing personalized consulting and training to NORC-H investigators that is not typically available in most NGS, genomics, bioinformatics, cell biology, or immunology core facilities. Following the retirement of Core Director Dr. Fred Ausubel (who has been the Director of Genomics Core since the original inception of the Center grant since 1994), Dr. Alessio Fasano, a renowned Celiac disease researcher and previously and Co-Core Director, has assumed the role of Director. The Co-Associate Director (Bioinformatics and Next Generation Sequencing) Dr. Ruslan Sadreyev, and Co-Associate Director (Cell Biology) Dr. Haining Shi will remain in charge of respective parts of the Core. There are 5 specific Aims of the Core: 1) provide consultative services related to genomics, cell biology, and immunology research design; 2) provide space, technical personnel, and specific training and supervision for utilizing specialized techniques and equipment used in cell biology, immunology, and genomics research, including single-cell transcriptional and epigenomic profiling, assistance in the development of high-content high-throughput assays using automated microscopic imaging, fluorescent activated cell sorting, and development of animal models; 3) provide a variety of genomics-related services related to next-generation sequencing; 4) provide bioinformatic analysis of various types of high-throughput data, including bulk and single-cell RNA-Seq, ATAC-seq, ChIP- Seq, CUT&RUN, Hi-C, metagenomics, proteomics, as well as computational analysis of molecular pathways, networks and phenotypes; and 5) conduct enrichment tutorials and workshops in basic concepts in genomic, bioinformatic, cell biology, and nutrition and GI immunology-related techniques, and coordinate summer internships for undergraduates and medical students. This will be accomplished through the significant expertise of the Core Directors and staff, with the help of advanced instrumentation for bioinformatics analysis, most up-to-date next generation sequencing instruments, automated microscopy and imaging based high content screening assays, a variety of equipment for carrying out immunological, cell biology, and morphology- related assays.