Organoid Shared Resource

有机类共享资源-摘要 有机类共享资源的建立是为了向癌症中心成员提供访问 培养和进行三维有机物实验所需的试剂、设备和专业知识 模特们。有机化合物技术于2012年被引入冷泉港实验癌症中心 作者David Tuveson，其理论基础是这些三维文化概括了许多方面 原代肿瘤生物学比标准二维细胞系更真实。现在有18个癌症中心 成员实验室(47%的成员)在他们的研究中使用人类和老鼠的有机类化合物，有机类化合物已经 在40份癌症中心出版物或预印本中使用。为了支持有机化合物的日益增长的使用，新的共享 Resources于2020年推出，拥有专门的实验室空间和工作人员。有机类共享资源正在被 在本申请中建议作为新的癌症中心共享资源，由系主任卡米拉·多斯桑托斯领导， 博士和经理Dennis Plenker博士。共享资源为成员提供集中访问 生成和使用有机培养物的协议、方法培训和标准化培养 试剂，以及在任何一个二维单元上进行高通量化学筛选的设备 线条或器官培养。癌症中心成员将受益于访问人类有机化合物的生物库 已经过验证并得到很好描述的模型。共享资源将能够为癌症提供 中心成员因大量购买培养试剂而节省了大量成本，以及 内部生产介质添加剂的协议。有机化合物的使用已经促进了几个合作 癌症中心的项目符合癌症中心的战略优先事项，包括调查癌症健康 不同人群之间的差异，整合定量生物学家和湿凳生物学家，并扩大翻译 研究。

ORGANOID SHARED RESOURCE – ABSTRACT The Organoid Shared Resource was established to provide Cancer Center members with access to the reagents, equipment, and expertise needed to culture and perform experiments with three-dimensional organoid models. The organoid technology was introduced to the Cold Spring Harbor Laboratory Cancer Center in 2012 by David Tuveson, with the rationale that these three dimensional cultures recapitulate numerous aspects of primary tumor biology more faithfully than standard two-dimensional cell lines. There are now 18 Cancer Center member labs (47% of members) using human and murine organoids in their research, and organoids have been used in 40 Cancer Center publications or preprints. To support the growing use of organoids, the new Shared Resource was launched in 2020 with dedicated lab space and staff. The Organoid Shared Resource is being proposed in this application as a new Cancer Center Shared Resource, led by Faculty Head Camila dos Santos, Ph.D., and manager Dennis Plenker, Ph.D. The Shared Resource offers members centralized access to protocols for generating and working with organoid cultures, training in methods, and standardized culture reagents, as well as equipment for performing high-throughput chemical screens on either two dimensional cell lines or organoid cultures. Cancer Center members will benefit from access to a biorepository of human organoid models that have been validated and well characterized. The Shared Resource will be able to offer Cancer Center members substantial cost-savings due to bulk purchasing of culture reagents, and the development of protocols to produce media additives in-house. The use of organoids has already fostered several collaborative Cancer Center projects in line with Cancer Center strategic priorities, including investigating cancer health disparities in diverse populations, integrating quantitative and wet-bench biologists, and expanding translational research.