Tissue regulation of T cell function - Imaging Core

项目摘要/摘要-成像核心 宿主对病原体感染的反应并不局限于单个组织，因此维持体内平衡 免疫监视和保护性免疫反应的发展需要免疫中的细胞 系统不断地在全身巡逻，有效地跨越多个组织屏障。此外，在他们的目标中 免疫细胞往往必须找到针对特定组织的定制解决方案，以有效地识别和根除 病原体。因此，直接观察白细胞在淋巴组织中的黏附、迁移和通讯 而非淋巴组织的显微镜检查是最重要的实验方法之一。影像 核心(核心B)是根据PPG调查人员的明确需求建立的，旨在利用 其关键人员在提供设计方面的专业知识和专业设备方面拥有丰富的经验 以及对感染组织中效应器T细胞功能的体内成像研究。的主要任务 成像核心如下：1.提供最先进的成像能力，以进行特定的 局部免疫过程中白细胞黏附、迁移及细胞间相互作用的实验研究 在多达六个维度(即3D-空间、时间、颜色、荧光信号)中的响应。2.发展一种 用于多细胞活体成像的新型高光谱多光子显微镜。3.提供技术援助 用于对收集的图像数据进行分析。4.协助筹划活体动物研究 提供标准化的感染/炎症模型和定制设计的环境 活细胞培养物的产生、保存和活体显微镜分析的生物安全条件 移植的组织和感染的小鼠。这些核心已经非常有效地实现了其中的一些 通过提供既促进研究又促进研究人员之间合作的服务来实现目标，以及 通过开发新的活体动物模型和成像技术。

PROJECT SUMMARY/ABSTRACT – IMAGING CORE The host response to pathogen infections is not confined to a single tissue, thus maintenance of homeostatic immune surveillance and the development of protective immune responses require that cells in the immune system constantly patrol the entire body, efficiently crossing multiple tissue barriers. Furthermore, in their target sites, immune cells often have to find customized tissue-specific solutions to effectively identify and eradicate pathogens. As such, the direct observation of leukocyte adhesion, migration and communication in lymphoid and non-lymphoid tissues with microscopy is one of the most important experimental approaches. The Imaging Core (Core B) has been established based on a stated need of PPG investigators and is designed to draw on the considerable experience of its key personnel to provide expertise and specialized equipment in the design and execution of in vivo imaging studies of effector T cell functions in infected tissues. The main missions of the Imaging Core are as follows: 1. To provide state-of-the-art imaging capabilities in conducting specific experiments regarding leukocyte adhesion and migration as well as cell-cell interactions during local immune responses in as many as six dimensions (i.e. 3D-space, time, color, fluorescence signals). 2. To develop a novel hyperspectral multiphoton microscopy for multicellular in vivo imaging. 3. To provide technical assistant for analysis of the collected image data. 4. To provide assistance in the planning of in vivo animal studies by offering standardized infectious/inflammation models and a custom-designed environment with appropriate biosafety conditions for the generation, housing and intravital microscopy analysis of live cell cultures, explanted tissues, and infected mice. The Cores have already been highly effective in achieving some of these goals by providing service that both facilitates research and fosters collaborations between investigators, and by enabling development of novel in vivo animal models and imaging techniques.