Human Tissue Core

偏头痛是最常见的原发性头痛疾病之一，影响四分之一的美国家庭。这个复杂 神经障碍部分由三叉神经躯体感觉的改变介导，三叉神经躯体感觉的改变表现为头/发- 坐骨神经痛和/或三叉神经异常性疼痛。偏头痛的有效治疗方法仍然有限，我们对偏头痛的认识 人三叉神经系统在基线和偏头痛条件下是稀疏的。响应RFA-NS-22-018，HEAL 计划：人类疼痛相关基因和细胞的发现和功能评价，我们建议 成立宾州人类精确疼痛中心（Penn HPPC），以阐明分子，细胞，表观遗传， 在基线和偏头痛条件下的人三叉神经节（TG）感觉神经元的生理概况。 Penn HPPC将由具有多学科专业知识的Penn和国际研究人员组成。的 PI，两个MPI和两个co-I目前正在合作进行人类背根的单细胞体深层RNA测序 神经节（DRG）项目，为该应用奠定了坚实的基础。Penn HPPC将包含三个 核心，并执行三个项目。其中，人体组织核心将作为唯一的实体， 获取和储存人体组织，并在研究项目中分配人体组织。Penn HPPC 人体组织获取和处理中心还将与其他U19中心和NIH HEAL协调 PRECISION人类疼痛网络倡议，用于人体组织采购、共享和SOP（标准操作程序） （程序）开发。人体组织核心将由PI罗博士领导，并得到她的研究支持 助理于医生和协调员人体组织核心还将包括两名宾夕法尼亚大学神经外科医生阿里博士 Zarina和Eric Zager，以及神经外科的协调员。特别是人体组织核心 将与不同的人体组织采购中心协调，包括其他U19的人体组织核心 中心，用于采购人类三叉神经节（TG）和背根神经节（DRG）组织，执行质量 控制所有采购的组织，储存和存档组织，在三个研究中心之间分发组织， 确保与人体组织相关的合规性和培训，并与行政核心合作， 向HEAL社区和医疗机构提供与人类TG和DRG相关的优化方案和程序。 疼痛研究领域。与Penn HPPC的其他组件一起，人体组织采购和 处理核心将有助于实现宾夕法尼亚州HPPC的拟议目标以及 NIH HEAL Initiative PRECISION人类疼痛网络。

Migraine, one of the most common primary headache disorders, affects 1 in 4 US households. This complex neurologic disorder is mediated in part by alterations in trigeminal somatosensation, which manifests as head/fa- cial pain and/or trigeminal allodynia. Effective treatments for migraine are still limited, and our knowledge about human trigeminal system at baseline and migraine conditions are sparse. In response to RFA-NS-22-018, HEAL Initiative: Discovery and Functional Evaluation of Human Pain-associated Genes & Cells, we propose to form the Penn Human Precision Pain Center (Penn HPPC) to elucidate molecular, cellular, epigenetic, and physiological profiles of human trigeminal ganglion (TG) sensory neurons at baseline and migraine conditions. The Penn HPPC will be composed of Penn and international investigators with multidisciplinary expertise. The PI, two MPIs, and two co-Is are currently collaborating on a single-soma deep RNA-seq of human dorsal root ganglion (DRG) project, which form a strong foundation for this application. The Penn HPPC will contain three cores and perform three projects. Among them, the human tissue core will function as the sole entity for procuring and storing human tissues and distributing human tissues among research projects. The Penn HPPC human tissue procurement and processing core will also coordinate with other U19 centers and the NIH HEAL Initiative PRECISION Human Pain network for human tissue procurement, sharing, and SOP (Standard Operat- ing Procedure) development. The human tissue core will be led by the PI, Dr. Luo, and supported by her research associate, Dr. Yu, and a coordinator. The human tissue core will also include two UPenn neurosurgeons, Drs Ali Zarina and Eric Zager, and a coordinator in the department of neurosurgery. Specifically, the human tissue core will coordinate with different human tissue procurement sites, including the human tissue cores of other U19 centers, for procuring human trigeminal ganglion (TGs) and dorsal root ganglion (DRG) tissues, perform quality control of all procured tissues, stock and archive the tissues, distribute tissues among the three research pro- jects, ensure compliances and trainings related to human tissues, and work with the administrative core to dis- seminate optimized protocols and procedures related to human TGs and DRGs to the HEAL community and the pain research field. Together with other components of the Penn HPPC, the human tissue procurement and processing core will help to achieve the proposed goals of Penn HPPC as well as the overarching goal of the NIH HEAL Initiative PRECISION Human Pain network.