Spatial genetics investigation of multinucleated cells -Administrative Supplement - Equipment

项目摘要/摘要 PI目前持有NIGMS R35研究奖1R35GM147209(空间遗传学 多核细胞的研究)。根据这项裁决，私家侦探要求补充 设备资金将用于购买MERSCOPE，第一个也是唯一一个商业 多重错误稳健荧光原位杂交技术平台， 它允许同时对数百种mrna进行单分子成像。 数十万个细胞。MERSCOPE平台还提供了一套软件工具 以及优化的试剂，将整个实验过程从样品简化到高质量 空间转录组数据。因此，它减少了人为干预和程序错误。这个 MERSCOPE系统将作为母公司R35项目的主力。该计划的主要目标是 资助的R35赠款是对基本遗传机制进行深入的空间研究 在巨大的合胞细胞中运作，就像肌纤维一样。这些独特的蜂窝系统包含 成百上千个细胞核，目前对基因的相互作用和协调知之甚少 在大量多核细胞中的表达程序。这一行政副刊，如果获得批准， 将促进MERSCOPE平台的购买，这将对成功发挥关键作用 受资助的R35项目。机构支持是用准备好购买的资金获得的 在父R35项目的整个生命周期内签订服务合同。

Project Summary/Abstract The PI holds a current NIGMS R35 research award 1R35GM147209 (Spatial genetics investigation of multinucleated cells). Under this award, the PI is requesting supplemental equipment funds that will be used to purchase MERSCOPE, the first and only commercial platform for Multiplexed Error-Robust Fluorescence in situ Hybridization (MERFISH) technology, which allows the simultaneous single-molecule imaging of hundreds of mRNA species in hundreds of thousands of cells. The MERSCOPE Platform also offers a suite of software tools and optimized reagents that streamline the whole experiment process from sample to high-quality spatial transcriptome data. It thus reduces human interventions and procedural errors. The MERSCOPE system will serve as the workhorse for the parent R35 project. The main goal of the funded R35 grant is to perform in-depth spatial investigations of the basic genetic mechanism operated in gigantic syncytial cells like myofibers. These unique cellular systems contain hundreds of nuclei and currently little is known about the interplay and coordination of gene expression programs in heavily multinucleated cells. This administrative supplement, if granted, will facilitate the purchase of a MERSCOPE Platform, which will serve a critical role in the success of the funded R35 project. The Institutional supports were acquired with funds ready to purchase service contracts throughout the life of the parent R35 project.