Identification of fish pathogens in California

项目摘要/摘要 加州大学动物健康和食品安全(CAHFS)系统 加州戴维斯兽医学院(SVM)由四个实验室组成 致力于兽医诊断学。作为美国协会的认可会员 兽医实验室诊断员(AAVLD)，CAHFS坚持严格的标准和 严格监督所有标准操作程序，以确保结果准确， 可重复的、一致的。临床样本和食品基质的微生物检测是不可或缺的 这是我们核心任务的一部分。CAHFS作为国家兽医诊断实验室提供服务 用于常规临床诊断、监管检测和食品的食用动物工业 病原体监测。对鱼类品种的诊断测试的需求正在增加， CAHFS目前正在建设能力，以加强鱼类和水产养殖测试， 出价了。其中主要感兴趣的领域是对细菌生物体的正确识别 与鱼病有关，以及抗菌素敏感性试验(AST)，或检测 考虑到生长面临的挑战，抗菌素耐药基因可作为体外AST的替代 大多数鱼类病原体在常规养殖条件下和临床上普遍缺乏 鱼中进行AST的断点和实验室标准。CAHFS之前没有 获得了能力建设资金，并正在申请这个机会，以种植我们的鱼类诊断 程序，包括细菌WGS测序能力，定制鱼的开发 病原菌MALDI-TOF文库加强对鱼类细菌的快速准确鉴定 临床样本中的微生物和基因组AMR监测。此外，由于有能力， 在微生物病原体上进行WGS，CAHFS将能够更好地对动物做出贡献 和食品病原体测序工作，并参与Vet-LIRN计划。美国卫生与公众服务部 该系统是唯一有资格协助兽医实验室调查响应的系统 网络(Vet-LIRN)任务，并处于监测 与加州食品和农业部协调的家畜 抗菌药物使用和管理计划。

PROJECT SUMMARY/ABSTRACT The California Animal Health and Food Safety (CAHFS) system, within the University of California, Davis School of Veterinary Medicine (SVM), is comprised of four laboratories dedicated to veterinary diagnostics. As an accredited member of the American Association of Veterinary Laboratory Diagnosticians (AAVLD), CAHFS adheres to rigorous standards and maintains strict oversight of all standard operating procedures to ensure results are accurate, repeatable, and consistent. Microbial testing of clinical samples and food matrices is an integral part of our core mission. CAHFS functions as the state veterinary diagnostic laboratory serving the food animal industries for routine clinical diagnostics, for regulatory testing and for food pathogen monitoring. The demand for diagnostic testing for fish species is increasing and CAHFS is currently building capacity to enhance the fish and aquaculture testing that can be offered. Among the major areas of interest are correct identification of bacterial organisms associated with fish disease, as well as antimicrobial susceptibility testing (AST), or detection of antimicrobial resistance genes as an alternative to in vitro AST, given the challenges with growth of most fish pathogens under routine culture conditions and the general lack of clinical breakpoints and laboratory standards for performing AST in fish. CAHFS has not previously received capacity building funding and is applying for this opportunity to grow our fish diagnostic program, including bacterial WGS sequencing capability, the development of a custom fish pathogen MALDI-TOF library to enhance rapid and accurate identification of fish bacterial organisms from clinical samples, and genomic AMR surveillance. Furthermore, with the capacity to perform WGS on microbial pathogens, CAHFS would be better able to contribute to animal and food pathogen sequencing efforts and participate in Vet-LIRN programs. The CAHFS system is uniquely qualified to assist with the Veterinary Laboratory Investigation Response Network (Vet-LIRN) mission and is at the forefront of monitoring antimicrobial resistance among livestock animals in coordination with the California Department of Food and Agriculture’s Antimicrobial Use and Stewardship program.