土壤盐渍化严重降低作物生产力，是当今世界农业面对的巨大难题。培育耐盐作物品种是解决这一难题的最高效方式。本课题组利用八倍体小冰麦PSR3628（美国引进）与国内外小麦品种杂交，筛选出耐盐小冰麦新种质（Ast191株系）。目前，国内外关于耐盐小冰麦的FISH核型及盐胁迫应答转录组分析领域的研究鲜见报道。本项目拟采用表型调查、SLAF-seq、RNA-seq和顺序mGISH–mFISH等方法分析耐盐小冰麦新种质的染色体组成与FISH特征，探究小麦与冰草属间的同源性及进化关系，小冰麦盐胁迫应答的代谢途径和关键候选基因，并将关键候选基因进行染色体定位。本项目的实施将有利于创造耐盐小冰麦新种质，为我国小麦新品种的培育提供资源支撑，对小麦遗传进化、盐胁迫应答关键基因克隆及分子机制等深入研究具有重要意义。

Soil salinization seriously reduces crop productivity, which is a huge problem for world agriculture today. Breeding crops with salt tolerance is the most efficient way to solve this problem. The octoploid Triticum-Agropyron PSR3628 (introduced from America) was hybridized with world wheat varieties, and new germplasm (Ast191 line) with salt tolerance has been selected by our research group. At present, there are few reports about the FISH karyotype and transcriptome analysis of salt stress-responsive in this new germplasm at home and abroad. Morphological observation, SLAF-seq, RNA-seq and sequential mGISH-mFISH etc, will be adopted to investigate chromosomal constitution and FISH pattern in these salt-tolerant germplasm, and understand homology and evolution relationship between Triticum and Agropyron. The metabolic pathway， key candidate genes involved in the salt stress response and chromosomal localization of these genes will be analyzed. The implementation of this project will be of great significance to produce salt-tolerant wheat germplasm for wheat breeding in China, and for wheat genetics, evolution, key gene cloning and molecular mechanism of salt stress response.