Increasing SARS-CoV-2 (COVID-19) testing capacity at the Nebraska Veterinary Diagnostic Center

项目摘要/摘要 内布拉斯加兽医诊断中心(NVDC)是一家提供全方位服务的兽医诊断中心 实验室，是美国兽医实验室协会完全认可的 诊断师，是FDA-CVM VET-LIRN网络的成员，是国家动物卫生组织 实验室网络(NAHLN)一级实验室，是国家认证实验室 家禽改良计划检测。该实验室每年处理超过300,000个样本 来自各种物种，包括牲畜、同伴动物、家禽、野生动物和动物园 动物。作为加强动物体内SARS-CoV-2监测的合作努力的一部分， 在2022-2023年间，超过3600只动物进行了不同动物种类的测试。为了增加 SARS-CoV-2在动物身上的诊断测试能力和能力，NVDC正在寻求 购买数字聚合酶链式反应系统的资金。该设备将使NVDC能够增强 SARS-CoV-2诊断测试允许极其敏感地检测SARS-CoV-2 动物标本。此外，数字聚合酶链式反应方法允许对SAR- 临床样本中的CoV-2基因组，这将有助于临床医生和One Health合作伙伴在 评估病毒水平和在动物中的传播。

Project Summary/Abstract The Nebraska Veterinary Diagnostic Center (NVDC) is a full-service veterinary diagnostic laboratory, is fully accredited by the American Association of Veterinary Laboratory Diagnosticians, is a member of FDA-CVM Vet-LIRN network, is a National Animal Health Laboratory Network (NAHLN) Level 1 laboratory, and is a certificated laboratory for National Poultry Improvement Plan testing. The laboratory processes over 300,000 specimens annually from a variety of species including livestock, companion animals, poultry, wildlife, and zoo animals. As part of a collaborative effort to enhance surveillance for SARS-CoV-2 in animals, more than 3,600 animals diverse animal species were tested in 2022-2023. To increase the diagnostics testing capacity and capabilities for SARS-CoV-2 in animals, the NVDC is seeking funds to purchase a digital PCR system. This equipment will enable the NVDC to enhance SARS-CoV-2 diagnostic testing by allowing extremely sensitive detection of SARS-CoV-2 in animal specimens. Additionally, the digital PCR approaches allows for quantification of SAR- CoV-2 genomes in clinical samples, which will help inform clinicians and One Health partners in assessing virus levels and transmission in animals.