Recombinant production of structured fats that mimic human milk fat

摘要 母乳是婴儿的首选营养，也是母乳的脂肪成分(母乳 脂肪，HMF)提供婴儿所需的最高比例的饮食能量。人类体内的脂肪 母乳是三酰甘油(TAG)，具有独特的立体异构体结构，有助于高效 婴儿肠道中的营养吸收。相比之下，植物油是人体内脂肪的主要来源。 商业婴儿配方奶粉：植物油中脂肪酸的组成和分布如下 与母乳中的不同，母乳中的营养会阻碍婴儿的营养吸收，因此他们的 成长。为了模仿人类乳脂的营养功能，人类乳脂替代品(HMF)有 是近年来通过酶促酯交换反应开发的。人乳脂 通过体外酶催化纯化植物油生产的替代品(HMF)具有所需的 高性能，但制造成本高，具有挑战性。雅利生物计划使用其重组的 酵母平台，为高质量的HMF提供具有成本效益的生物制造解决方案。我们 建议使用我们的平台高效地生产与人类乳脂相似的结构性脂肪 物理化学性质，并产生高产、专有的积累脂肪的酵母菌株 具有类似于人奶脂肪的立体异构体组成。这里描述的方法应该是 导致了一种用于重组结构脂肪的成本效益高的生物制造方法， 模仿人类乳脂。

Abstract Human breast milk is the choice nutrition for infants, and the fat component of the milk (human milk fat, HMF) supplies the highest fraction of the infant's required dietary energy. The fats within human breast milk are triacylglycerols (TAGs) with a unique stereoisomeric structure that facilitates efficient nutrient absorption in the infant gut. In contrast, vegetable oils form the main source of fats in commercial infant formulas: the composition and distribution of the fatty acids in vegetable oils are different from those in human milk which hinders nutrient absorption in infants, and consequently their growth. To mimic the nutritional functions of human milk fat, human milk fat substitutes (HMFS) have been developed through enzymatic interesterification reactions in recent years. Human milk fat substitutes (HMFS) produced by in vitro enzyme-catalysis of purified vegetable oils, have the desired properties but are challenging to manufacture with high cost. Yali Bio aims to use its recombinant yeast platform to provide a biomanufacturing solution for high quality HMFS that is cost-effective. We propose to use our platform to efficiently produce structured fats that resemble human milk fat in their physicochemical properties and generate highly productive, proprietary yeast strains accumulating fats with a stereoisomeric composition similar to human milk fats. The approach described here should lead to a cost-effective biomanufacturing method for recombinant production of structured fats that mimic human milk fats.