GNOTOBIOTIC RATS TO STUDY BREAST VERSUS FORMULA MILKS

第1 - 3天，接种无菌大鼠（4组，每组每种性别6只） 母乳喂养婴儿的粪便 每个小组将被安置在一个单独的 正压隔离器 从第1天开始，动物将接受以下喂养： 1)人类母乳，2）Enfamil（tm）配方奶粉，3）Enfamil（tm）牛奶 补充有低聚果糖（FOS：5%wt/wt）的配方食品和4） 标准食物。 在第1天（接种前）、第5天、第9天和第13天新鲜排泄的粪便 将从每只动物中收集细菌、乳酸杆菌 梭菌会被培养并计数 在第17天， 杀了 它们的后肠内容物将被移除并进行如下测试：1） pH值，2）含水量，3）细菌细胞脂肪酸，4）短链 脂肪酸，5）胆汁酸代谢，6）中性固醇代谢，7）四 细菌酶（β葡糖苷酶、β糖苷酶、偶氮还原酶和 硝基还原酶）和8）10个关键物种的定量PCR探测，以及 最后，9）在12选择性和/或鉴别培养基上的细菌计数。 FOS如何将配方奶喂养的植物群转化为更理想的母乳喂养 将通过比较结果来评估对应方。 拟议的商业应用;接种人粪便的无菌大鼠， 来自婴儿或成人的，将用作体内系统来筛选任何 口服化合物，从婴儿配方奶粉到olestra， 抗生素到纤维

On days 1 to 3 germfree rats (4 groups, 6 per sex per group) will be seeded with feces from breast fed babies. Each group will be housed in a separate positive pressure isolator. From day 1 animals will be fed the following; 1) human breast milk, 2) Enfamil(tm) milk formula, 3) Enfamil(tm) milk formula supplemented with fructooligosaccharides (FOS: 5% wt/wt) and 4) standard chow. On days 1 (pre-seeding), 5, 9 and 13 freshly voided feces from each animals will be collected and the bifidobacteria, lactobacilli and clostridian will be grown and counted. On day 17 the rats will be killed. Their hindgut contents will be removed and tested as follows: 1) pH, 2) percent water, 3) bacterial cellular fatty acids, 4) short chain fatty acids, 5) bile acid metabolism 6) neutral sterol metabolism, 7) four bacterial enzymes (beta glucosidase, beta glycosidase, azoreductase and nitroreductase) and 8) quantitative PCR probing for 10 key species, and lastly, 9) bacterial counts on a 12 selective and/or differential media. How well FOS convert a formula-fed flora to its more desirable breast-fed counterpart will be assessed by comparing the results. PROPOSED COMMERCIAL APPLICATION; Germfree rats seeded with human feces, from an infant or an adult, will be used as an in vivo system to screen any orally ingested compound, from infant milk formula to olestra, from antibiotics to fiber.