More Than Mechanical Retention: Characterization of Lactobacillus Clinical Strains Using In Vitro Models

不仅仅是机械保留：使用体外模型表征乳酸菌临床菌株

• 批准号: 10593599
• 负责人: ZEZHANG TOM WEN
• 金额: $ 22.28万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10593599
• 关键词: Acids; Adopted; Amino Acids; Anabolism; Bacteria; Bacterial Adhesins; Biological Assay; Caring; Clinical; Code; Collection; Communicable Diseases; Communities; Complex; Consumption; Cues; Dental Plaque; Dental caries; Development; Disease; Ecology; Emergency Situation; Etiology; Foundations; Functional disorder; Genes; Glucans; Glucosyltransferase; Goals; Health; Hour; Hydrogen Peroxide; In Vitro; Indigenous; Inverse Polymerase Chain Reaction; Investigation; Knowledge; Lactobacillus; Lactobacillus casei; Lead; Lesion; Libraries; Link; Mechanics; Mediating; Metabolic; Microbial Biofilms; Modeling; Mutagenesis; Oral; Oral cavity; Outcome; Pathogenicity; Peptides; Polysaccharides; Production; Proteins; Role; Saliva; Site; Source; Streptococcus mutans; Sucrose; Surface; Testing; antagonist; bacterial metabolism; bacteriocin; cost; dental biofilm; dysbiosis; genetic regulatory protein; in vitro Model; insight; member; metabolomics; microbiota; mutant; novel; novel strategies; pathogen; productivity loss; response; stressor; sugar; tooth surface

SUMMARY Dental caries remains among the most prevalent infectious disease worldwide, costing USA tens of billions of dollars annually in associated care and productivity losses. Dental caries is a result of imbalance of the indigenous microbiota on the tooth surface known as dental plaque, and the acidic metabolites of bacterial metabolism are directly linked to the disease development. It is well understood that conditions like continuous consumption of fermentable sugars, especially sucrose and saliva deficiency favor the emergency of a highly acidogenic and aciduric plaque microbiota, which include Streptococcus mutans and lactobacilli. Lactobacillus (Lb) sp. were the first implicated in dental caries over a century ago, but major gaps remain in knowledge concerning how the different Lb sp. establish and persist in the plaque microbiota and promote the development of carious lesions. In this study, a collection of hundreds of different clinical Lb isolates will be examined using well established in vitro models to elucidate how major groups of Lb sp. colonize and establish on a surface; how S. mutans and environmental conditions may modulate the ability of major Lb sp. to establish and compete in a mixed-species consortium; and how interactions between S. mutans and major Lb sp. influence the community dynamics under conditions typical of the oral cavity. It is highly anticipated that the results from this study will lead to novel insights on each of these aspects and the roles of oral Lb in plaque microbiota and dental caries. Together, the information derived from this study should also serve as a foundation for further investigation on the pathophysiology of oral Lb, the plaque biofilm ecology, and the development of novel strategies against dental caries and other conditions where dysbiotic microbiota is a major underlying factor.

总结 龋齿仍然是世界范围内最流行的传染病之一，给美国造成数百亿美元的损失 每年在相关的护理和生产力方面的损失。龋齿是由于牙齿的不平衡， 牙齿表面上的固有微生物群称为牙菌斑，以及细菌的酸性代谢产物 代谢与疾病的发展直接相关。众所周知， 持续消耗可发酵糖，特别是蔗糖和唾液缺乏有利于 高度产酸和耐酸菌斑微生物群的紧急情况，包括变形链球菌和 乳酸杆菌。乳酸杆菌（Lactobacillus，Lb）是世纪前第一个与龋齿有关的细菌，但主要是乳酸杆菌（Lactobacillus，Lb）。 关于不同的Lb如何在斑块中建立和持续存在的知识仍然存在空白 微生物群和促进龋齿病变的发展。在这项研究中，收集了数百个 将使用完善的体外模型检查不同的临床LB分离株，以阐明其主要作用 LB sp.群体定殖并建立在表面上; S.变异体和环境条件可以 调节主要Lb sp.建立和竞争混合物种财团的能力;以及如何 S之间的相互作用。在一定条件下，变形杆菌和主要Lb sp. 典型的口腔。人们高度期待这项研究的结果将导致对以下方面的新见解： 这些方面中的每一个以及口腔Lb在菌斑微生物群和龋齿中的作用。统称 从这项研究中获得的信息也应该作为进一步调查的基础。 口腔Lb的病理生理学，菌斑生物膜生态学，以及针对Lb的新策略的开发 龋齿和其他病症，其中生态失调的微生物群是主要的潜在因素。