More Than Mechanical Retention: Characterization of Lactobacillus Clinical Strains Using In Vitro Models

不仅仅是机械保留：使用体外模型表征乳酸菌临床菌株

• 批准号: 10593599
• 负责人: ZEZHANG TOM WEN
• 金额: $ 22.28万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10593599
• 关键词: Acids; Adopted; Amino Acids; Anabolism; Bacteria; Bacterial Adhesins; Biological Assay; Caring; Clinical; Code; Collection; Communicable Diseases; Communities; Complex; Consumption; Cues; Dental Plaque; Dental caries; Development; Disease; Ecology; Emergency Situation; Etiology; Foundations; Functional disorder; Genes; Glucans; Glucosyltransferase; Goals; Health; Hour; Hydrogen Peroxide; In Vitro; Indigenous; Inverse Polymerase Chain Reaction; Investigation; Knowledge; Lactobacillus; Lactobacillus casei; Lead; Lesion; Libraries; Link; Mechanics; Mediating; Metabolic; Microbial Biofilms; Modeling; Mutagenesis; Oral; Oral cavity; Outcome; Pathogenicity; Peptides; Polysaccharides; Production; Proteins; Role; Saliva; Site; Source; Streptococcus mutans; Sucrose; Surface; Testing; antagonist; bacterial metabolism; bacteriocin; cost; dental biofilm; dysbiosis; genetic regulatory protein; in vitro Model; insight; member; metabolomics; microbiota; mutant; novel; novel strategies; pathogen; productivity loss; response; stressor; sugar; tooth surface

SUMMARY Dental caries remains among the most prevalent infectious disease worldwide, costing USA tens of billions of dollars annually in associated care and productivity losses. Dental caries is a result of imbalance of the indigenous microbiota on the tooth surface known as dental plaque, and the acidic metabolites of bacterial metabolism are directly linked to the disease development. It is well understood that conditions like continuous consumption of fermentable sugars, especially sucrose and saliva deficiency favor the emergency of a highly acidogenic and aciduric plaque microbiota, which include Streptococcus mutans and lactobacilli. Lactobacillus (Lb) sp. were the first implicated in dental caries over a century ago, but major gaps remain in knowledge concerning how the different Lb sp. establish and persist in the plaque microbiota and promote the development of carious lesions. In this study, a collection of hundreds of different clinical Lb isolates will be examined using well established in vitro models to elucidate how major groups of Lb sp. colonize and establish on a surface; how S. mutans and environmental conditions may modulate the ability of major Lb sp. to establish and compete in a mixed-species consortium; and how interactions between S. mutans and major Lb sp. influence the community dynamics under conditions typical of the oral cavity. It is highly anticipated that the results from this study will lead to novel insights on each of these aspects and the roles of oral Lb in plaque microbiota and dental caries. Together, the information derived from this study should also serve as a foundation for further investigation on the pathophysiology of oral Lb, the plaque biofilm ecology, and the development of novel strategies against dental caries and other conditions where dysbiotic microbiota is a major underlying factor.

概括 龋齿仍然是全球最普遍的传染病之一，耗资美国数十亿美元 每年在相关的护理和生产力损失中的美元。龋齿是由于不平衡的结果 牙齿表面上的土著菌群称为牙菌斑，细菌的酸性代谢产物 代谢与疾病发展直接相关。众所周知，条件像 连续食用可发酵糖，尤其是蔗糖和唾液缺乏症有利于 高度酸性和酸性斑块菌群的紧急情况，其中包括突变链球菌和 乳杆菌。乳杆菌（LB）sp。是一个世纪前的第一个与龋齿有关，但很重要 关于不同LB SP的知识，缺口仍然存在。建立并持续存在 微生物群并促进龋齿病变的发展。在这项研究中，数百个集合 将使用良好的体外模型检查不同的临床LB分离株，以阐明如何主要 lb sp。组。在表面上定居并建立； S. Mutans和环境条件如何 调节主要LB sp的能力。建立和竞争混合物联盟；以及如何 S. mutans和主要LB sp之间的相互作用。在条件下影响社区动态 典型的口腔。备受期待的，这项研究的结果将导致对 这些方面中的每一个以及口服LB在斑块微生物群和龋齿中的作用。在一起， 从这项研究中得出的信息也应为进一步调查的基础 口服LB的病理生理，牙菌膜生态学以及针对的新型策略的发展 龋齿和其他疾病，其中不植物菌群是主要的基本因素。