CNS MEDIATION OF THE AIRWAY RESPONSE TO LUNG INFLATION

一个大聋子知道负责的传入和传出通路 调解Hering-Breuer通胀反射，但人们知之甚少 关于参与这种反射的中枢神经系统通路 弧形。Hering-Breuer充气反射对呼吸的影响 节律发生和心血管控制已被广泛研究， 而这种反射对气道张力的影响在很大程度上被忽视了。 拟议的研究旨在阐明中枢通路 调节呼吸道对Hering-Breuer充气反射的反应。三 中央通路，已被记录为在 中枢性呼吸控制，将在 建议进行的研究。这些途径将用g化学进行研究。 刺激神经元胞体以确定是否激活 潜在的中继站以一种模仿 Hering-Breuer反射的激活。突触传递将是 在潜在的中继点被暂时阻止，以确定此动作 减弱或防止对肺充气的反射性呼吸道反应。 将使用解剖示踪剂来确认这些 Hering-Breuer反射弧线上的潜在中继点。胞外 单细胞记录将与逆行扫描结合使用 刺激支配呼吸道的迷走神经分支以允许 迷走神经自发放电模式的特征(S) 气管运动和支气管运动节前神经元。这些录音将会 也允许表征这些迷走神经的放电反应 节前神经元对肺膨胀的影响 潜在中继点的突触传递。

A great deaf is known about the afferent and efferent pathways responsible for mediating the Hering-Breuer Inflation reflex, but very little is known about the central nervous system pathways that are involved in this reflex arc. The influence of the Hering-Breuer Inflation reflex on respiratory rhythmogenesis and cardiovascular control has been studied extensively, while the influence of this reflex on airway tone has largely been ignored. The proposed studies are designed to elucidate the central pathways mediating the airway response to the Hering-Breuer Inflation reflex. Three central pathways, which have been documented as being significant in central respiratory control, will be examined during the course of the proposed studies. These pathways will be studied using g chemical stimulation of neuronal cell bodies to determine if the activation of potential relay sites reduces airway tone in a manner which mimics the activation of the Hering-Breuer reflex. Synaptic transmission will be blocked transiently at potential relay sites to determine if this maneuver attenuates or prevents the reflex airway response to lung inflation. Anatomical tracers will be used to confirm the participation of these potential relay sites in the Hering-Breuer reflex arc. Extracellular single-cell recording will be used in conjunction with antidromic stimulation of vagal branches that innervate the airway to permit the characterization of the spontaneous discharge pattern(s) of vagal tracheomotor and bronchomotor preganglionic neurons. These recordings will also permit the characterization of discharge response of these vagal preganglionic neurons to lung inflation before and during the blockade of synaptic transmission at the potential relay sites.