MATURATION OF DIAPHRAGM NEUROMUSCULAR TRANSMISSION

本研究项目的目的是研究神经肌肉的作用 在隔膜传动故障中，确定这种作用如何变化 随着成熟。 另外，我将确定神经肌肉 传输受慢性负荷的调节。 我的假设是 神经肌肉传递功能障碍在 在成年人中，甚至在成年人中， 新生儿 我的具体目标是：1）确定神经肌肉是否 在长期使用仪器的清醒成人体内发生传输故障 绵羊在急性吸气流量阻力负荷呼吸; 2）研究 急性期神经肌肉传递的成熟变化 在清醒的羔羊（年龄<1周龄，2 wk、4 wk和8 wk）; 3）测定慢性吸气流量 电阻性负荷改善了膈肌中的神经肌肉传递， 成年和新生绵羊.运动功能指数的测定 和体内神经肌肉传递（Pdi，EMG，单个单位动作 电位、膈神经图、通气、血气）将使用 最先进的技术 由于该提案提出了一个综合的 神经肌肉传递功能障碍的研究进展 年龄的增长，很可能会产生新的信息， 直接关系到我们对呼吸肌功能的理解， 早年的失败

The purpose of this research project is to study the role of neuromuscular transmission failure in the diaphragm and determine how this role changes with maturation. In addition, I will determine how neuromuscular transmission is modulated by chronic loading. My hypothesis is that neuromuscular transmission failure plays a significant role in the development of diaphragmatic fatigue in adults, and even more so in newborns. My specific aims are to: 1) determine whether neuromuscular transmission failure occurs in-vivo in chronically instrumented awake adult sheep during acute inspiratory flow resistive loaded breathing; 2) study the maturational changes in neuromuscular transmission during acute inspiratory flow resistive loaded breathing in awake lambs (ages <1 wk, 2 wk, 4 wk and 8 wk) ; 3) determine whether chronic inspiratory flow resistive loading improves neuromuscular transmission in the diaphragm in adult and newborn sheep, Measurements of indices of diaphragmatic function and neuromuscular transmission in-vivo (Pdi, EMG, single unit action potentials, phrenic neurogram, ventilation, blood gases) will be done using state-of-the-art techniques. Since this proposal presents an integrated approach to the study of neuromuscular transmission failure ad a function of age, it is highly likely to generate new information which will have direct relevance to our understanding of respiratory muscle function and failure in early life.