DELIVERY OF ANTIOXIDANT ENZYMES & GENES TO NEONATAL LUNG

描述：（改编自研究者摘要）：目标1将确定 保护细胞免受 高氧。这将使用肺上皮细胞培养系统来完成 暴露在高氧环境中目的2是改善AOE在小鼠体内的分布和摄取， 肺将在细胞培养物和小鼠中检测稳定的混悬液 暴露于高氧对细胞活力，病毒感染性， 多基因递送和表达以及损伤程度。目标3将使用 含有受人SP-B控制的AOE基因的病毒构建体 启动子（以靶向II型或Clara细胞）或CMV启动子。目标4： 比较PFC蛋白或腺病毒相关AOE悬浮液对 正常或受伤，早产或足月羔羊肺，使用水平和 转基因表达的持续时间。

DESCRIPTION: (Adapted from the Investigator's Abstract): Aim 1 will determine the optimal combination of supplemental AOE required to protect cells from hyperoxia. This will be done using a pulmonary epithelial cell culture system exposed to hyperoxia. Aim 2 is to improve the distribution and uptake of AOE in the lung. Stabilized suspensions will be tested in cell culture and in mice exposed to hyperoxia for their effects on cell viability, virus infectivity, multiple gene delivery and expression, and degree of injury. Aim 3 will use viral constructs containing AOE genes under the control of the human SP-B promoter (to target Type II or Clara cells) or the CMV promoter. Aim 4 is to compare the effects of PFC-protein or adenoviral-associated AOE suspensions on normal or injured, preterm or term lamb lungs, using assays for levels and duration of transgene expression.