GENE TRANSFER TO THE VASCULAR ENDOTHELIUM

描述(来自申请的逐字)：这些研究的目标是 使用体内平移方法与噬菌体展示文库一起识别归巢 心脏新生血管、冠脉内皮细胞和心脏的多肽 心内膜。我们希望使用这些多肽，特别是原型RGD 新生血管归巢肽作为靶向逆转录病毒和HIV慢病毒的配体 把载体带到这些组织。我们还将研究选择更多内容的方法 来自组合逆转录病毒文库的高效RGD靶向载体。 最后，我们建议表征这些靶向药物的生物分布。 通过灵敏的荧光方法获得载体，并将载体附着与 血管内皮细胞特异性基因表达的位置。

DESCRIPTION (Verbatim from the application): The goal of these studies is to use in vivo panning methods with phage display libraries to identify homing peptide for the heart neovasculature, the coronary endothelium and the endocardium. We wish to use these peptides, especially the prototype RGD neovascular homing peptide, as ligands to target retrovirus and HIV lentivirus vectors to those tissues. We will also examine a method for selecting more efficient RGD-targeted vectors from a combinatorial retrovirus library. Finally, we propose to characterize the biodistribution of these targeted vectors by sensitive fluorescence methods and correlate vector attachment with sites of endothelial-specific gene expression.