An integrated platform for studying sensory networks in the vertebrate brain

7.项目摘要/摘要 人类的经验是由我们的感官塑造的，这些感官从我们的 环境。然而，这些不同的输入都构成了一个单一的 在我们心目中的外部世界的代表性。神经学家研究了感官 感知，以及整合不同的感觉形式，如视觉，听觉和 触摸，几十年了，但仍然有一些重要的悬而未决的问题 大脑中的感觉细胞工作，以及它们形成的回路如何控制 通过大脑传递信息。详细了解这些网络将扩大我们的 对大脑的一般知识，并将作为解决问题的起点 像自闭症谱系障碍这样的疾病，感觉系统功能异常。 在很大程度上，这些起作用的感觉回路对技术人员来说仍然是个谜。 理由。传统的技术要么从整个大脑取样而不看清 或一次记录几个神经元而看不到较大的 网络。该项目使用斑马鱼模型系统通过成像来解决这一差距 整个大脑的活动，包括每个神经元单独的活动。第一 AIM包含一个计划，在大脑感知和识别时绘制这种全脑活动图 处理视觉或听觉信息，使用一套新的感官方法 刺激。第二个目标中的实验将呈现各种视觉和听觉 同时刺激，寻找大脑功能网络的方式 整合这些信息。这些数据将用于构建数学网络模型 大脑如何处理和整合视觉和听觉。第三个目标将会消失 这些感觉神经元的功能和结构方面的光，揭示了生物学 我们将用来调整先前目标的纯数学模型的现实。 由此产生的生物基础模型将为混凝土功能 在第四个目标中的实验，我们将通过光基因激活或沉默特定的 电路元件来测试模型的预测。 总体目标是第一次描述大脑中的所有听觉和视觉神经元 大脑，并开发和测试它们如何接收、处理和整合的模型 通过感官通道的信息。

7. Project Summary/Abstract Human experience is shaped by our senses, which receive diverse inputs from our environment. These varied inputs, however, all contribute to a single integrated representation in our minds of the outside world. Neuroscientists have studied sensory perception, and the integration different sensory modalities like vision, hearing, and touch, for decades, but there are still important unanswered questions about how sensory cells in the brain work, and how the circuits that they form control the flow of information through the brain. Understanding these networks in detail would expand our knowledge of the brain in general, and would serve as a starting point for addressing disorders like autism spectrum disorder, in which sensory systems function abnormally. In a large part, these functioning sensory circuits have remained mysterious for technical reasons. Traditional techniques either sample from the whole brain without seeing the individual neurons or record from a few neurons at a time without seeing the larger networks. This project addresses this gap using the zebrafish model system by imaging activity across the entire brain, including the activity of each neuron individually. The first aim contains a plan to map such brain-wide activity while the brain perceives and processes visual or auditory information, using a set of novel approaches for sensory stimulation. Experiments in the second aim will present various visual and auditory stimuli simultaneously, looking for the ways in which the brain's functioning networks integrate this information. These data will be used to build mathematical network models of how the brain processes and integrates vision and hearing. The third aim will shed light on functional and structural aspects of these sensory neurons, revealing biological realities that we will use to adjust the purely mathematical models from the prior aims. The resulting biologically grounded models will set the stage for concrete functional experiments in the fourth aim, where we will optogenetically activate or silence specific circuit elements to test the models' predictions. The overall goal is to describe, for the first time, all of the auditory and visual neurons in the brain, and to develop and test models for how they receive, process, and integrate information across sensory modalities.