Study of Lead Exposure & Outcomes Amongst Children in C*

描述（由申请人提供） 本修订提案中的研究将主要在印度进行，作为NIH资助#R01 ES 07821的扩展。铅暴露是印度的一个主要环境健康问题。印度尚未进行直接研究，以调查铅暴露对神经行为发育等结果的影响。我们建议建立在拟议PI的工作期间发起的合作努力，作为富布赖特高级学者，并与印度泰米尔纳德邦的Sri Ramachandra医学院和金奈研究所（以前称为马德拉斯）的同事合作，研究750名4-6岁的儿童在金奈地区就读特定的公立学校。这些学校将被选择代表城市的高-高，高-低，低-高，低-低交通和工业方面的地区。对于每个孩子，我们将测量血液和脱落乳牙中的铅水平;评估视觉空间功能（使用Raven's Progressive Matrices），视觉功能（使用视觉运动能力的广泛评估），行为（使用Conners' Rating Scale-Revised）和IQ（使用Binet-Kamath检验）;采集血液用于提取DNA，并对氨基乙酰丙酸转氨酶（ALAD）、载脂蛋白E和转铁蛋白基因的多态性进行基因分型;并收集协变量数据。 有了这些信息，我们将描述铅暴露和剂量的关系，并测试特定的假设，铅剂量的神经行为结果的关系和修改这些相同的关系的遗传多态性的影响。这项研究有望提供新的科学信息，铅毒性，以及在印度城市的儿童神经行为发育铅暴露的影响的重要信息。它还将成为美国和印度之间建立合作环境健康研究的基地。

DESCRIPTION (provided by applicant) The research in this revised proposal will be performed primarily in India as an extension of NIH grant #R01 ES07821. Lead exposure poses a major environmental health problem in India. No direct studies have yet been performed in India to investigate the impact of lead exposure on outcomes such as neurobehavioral development. We propose to build upon a collaborative effort initiated during the proposed PI's work as a Senior Fulbright Scholar and work with colleagues at the Sri Ramachandra Medical College and Research Institute of Chennai (formerly known as Madras), Tamil Nadu, India, to study 750 children aged 4-6 attending specific public schools in the area of Chennai. These schools will be chose to represent areas of the city that are high-high, high-low, low-high, and low-low with respect to traffic and industry. For each child we will measure levels of lead in blood and shed primary teeth; assess visuospatial function (using Raven's Progressive Matrices), visuomotor function (using the Wide Range Assessment of Visual Motor Abilities), behavior (using Conners' Rating Scale-Revised), and IQ (using the Binet-Kamath test); collect blood for the extraction of DNA and genotyping for polymorphisms of the aminolevulinic acid dehydratase (ALAD), apoliproprotein E, and transferrin genes; and collect data on covariates. With this information we will describe lead exposure and exposure-dose relationships and test specific hypotheses on the relationship of lead dose to neurobehavioral outcomes and the modifying effect of genetic polymorphisms on these same relationships. This research promises to provide novel scientific information on lead toxicity as well as important information on the impact of lead exposure on pediatric neurobehavioral development in urban India. It will also serve as a nidus for building collaborative environmental health research between the U.S. and India.