CORE--Molecular Biology and Vector Core

分子生物学和载体核心(Core B)将具有以下功能：1)维护重组DNA载体的库存，2)进行大规模的质粒制备，3)进行质粒克隆，4)进行HEK 293细胞的转染，5)进行大规模的腺病毒制备，6)鉴定重组腺病毒(实验室和GMP级)，7)在每次患者注射后分析腺病毒的效力，8)使用PCR/Southern印迹分析监测患者血液中腺病毒DNA的存在。分子生物学和载体核心将为项目1和项目2提供实验室级CSCL梯度提纯的腺病毒。临床级(GMP)腺病毒将在贝勒基因载体实验室制造。临床级病毒在用于项目3之前，将在分子生物学和媒介核心中进行广泛的表征。

The Molecular Biology and Vector Core (Core B) will have the following functions: 1) maintain inventory of recombinant DNA vectors, 2)perform large-scale plasmid preps, 3) perform plasmid cloning, 4) perform HEK 293 cell transfections, 5) perform large-scale adenovirus preps, 6) characterize recombinant adenoviruses (laboratory and GMP grade), 7) analyze potency of adenovirus following each patient injection, 8) monitor presence of adenoviral DNA in patient's blood using PCR/Southern blot assay. The Molecular Biology and Vector Core will provide laboratory grade, CsCl gradient-purified adenovirus to Projects 1 and 2. Clinical grade (GMP) adenovirus will be manufactured at the Baylor Gene Vector Laboratory. Clinical grade virus will be characterized extensively in the Molecular Biology and Vector Core before it is used in Project 3.