Virus Production & Molecular Biology Core

病毒生产

• 批准号: 10705294
• 负责人: In-Jung Kim
• 金额: $ 22.52万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705294
• 关键词: Area; Cell Differentiation process; Cells; Consultations; Core Grant; Custom; DNA Modification Process; DNA Viruses; Dependovirus; Equipment; Gene Delivery; Genetic; Genetic Techniques; Genetically Modified Animals; Genotype; Histologic; Histological Techniques; Immunohistochemistry; Knowledge; Laboratories; Laboratory Personnel; Mediating; Modernization; Molecular; Molecular Biology; Molecular Biology Techniques; Mus; Neurons; Pathogenicity; Persons; Plasmids; Preparation; Production; Research; Research Personnel; Resources; Services; Source; Students; Techniques; Training; Transgenic Animals; Viral; Viral Vector; Virus; Vision; Vision research; adeno-associated viral vector; design; experience; immunogenicity; laboratory experience; programs; tool; transgene delivery; transgene expression

Virus Production & Molecular Biology Core Project Summary The Virus Production & Molecular Biology Core module will provide the means to incorporate modern molecular and genetic techniques into the programs of vision research investigators at Yale. First, the Core module will supply virus preparation assistance and related services for laboratories working with transgenic animals and/or viral techniques. Second, the Core module will train new investigators and students in basic molecular biology techniques, including DNA modification to generate new viral vectors, as well as histological techniques, such as immunostaining. Third, it provides and maintains a range of molecular biology equipment to be shared among vision researchers who lack such equipment in their own laboratories. Viral vectors are powerful tools for gene delivery in terminally differentiated cells. For example, adeno-associated viruses (AAVs) have enjoyed increasingly wide application in vision research based on their many favorable features that include the lack of pathogenicity, low immunogenicity, and the ability to maintain long-term transgene expression in neurons. The Virus Production & Molecular Biology Core module will be capable of providing both AAV vector design and production to meet a variety of research needs among vision researchers at Yale. An in-house capability for virus design and production will significantly enhance vision research by producing custom, high-titer AAV products not available from commercial sources. Consultation and training in molecular, genetic and histological techniques will expand the research capabilities of vision researchers that lack sufficient background in these areas with the convenience of in-person consultation for specific research needs.

病毒生产和分子生物学核心 项目摘要 病毒生产和分子生物学核心模块将提供将现代 分子和遗传技术的应用到耶鲁大学的视觉研究项目中。第一，核心 模块将为从事转基因工作的实验室提供病毒制备协助和相关服务 动物和/或病毒技术第二，核心模块将培训新的调查员和学生， 分子生物学技术，包括DNA修饰以产生新的病毒载体，以及 组织学技术，如免疫染色。第三，它提供并维持了一系列的分子 视觉研究人员之间共享生物设备，他们自己缺乏这种设备。 laboratories.病毒载体是在终末分化细胞中进行基因递送的有力工具。例如，在一个示例中， 腺相关病毒（adeno-associated viruses，AAV）在基于腺病毒的视觉研究中得到越来越广泛的应用。 它们具有许多有利的特征，包括缺乏致病性、低免疫原性和 维持神经元中的长期转基因表达。病毒生产与分子生物学核心 模块将能够提供AAV载体设计和生产，以满足各种研究 耶鲁大学视觉研究人员的需求。病毒设计和生产的内部能力将 通过生产定制高滴度AAV产品，显著增强了视觉研究， 商业来源。将扩大分子、遗传和组织学技术方面的咨询和培训 视觉研究人员的研究能力，缺乏足够的背景，在这些领域与 为特定研究需求提供面对面咨询的便利。