Core--In Vivo Procedures Core

该核心组件将评估项目中开发的试剂的急性毒性和增强中度高温对小鼠体内加热的肿瘤异种移植物的放射增敏作用的能力。核心还将确定与热诱导放射增敏（HIR）和/或其体外增强相关的参数（如项目中确定的）是否也与体内HIR或其增强相关。这项工作的长期目标是开发非侵入性成像这些参数的方法。简而言之，该核心将与项目研究人员合作，将其体外研究扩展到利用在裸鼠中作为异种移植物生长的人类肿瘤的初始临床前模型。 该核心的建立将为评估项目3和4中开发的化合物的体内功效和毒性提供有效且具有成本效益的资源。此外，核心将与所有项目合作，以确认有助于体外HIR增加的参数（即分子改变）也与体内HIR或其增强相关。

This Core Component will evaluate reagents developed in the Projects for acute toxicity and the ability to enhance the radiosensitizing effects of moderate hyperthermia in tumor xenografts heated in vivo in mice. The Core will also determine if the parameters that relate to heat-induced radiosensitization (HIR) and/or its enhancement in vitro (as established in the Projects) also relate to HIR or its enhancement in vivo. A long-range goal of this effort is to develo methods to image these parameters non-invasively. Briefly, this Core will collaborate with the Project researchers to extend their in vitro studies to initial pre-clinical models utilizing human tumors grown as xenografts in nude mice. The establishment of this Core will provide an efficient and cost effective resource for evaluating the in vivo efficacy and toxicity of compounds developed in Projects 3 and 4. In addition, the Core will collaborate with all of the Projects to confirm that the parameters (i.e. molecular alterations) that contribute to increased HIR in vitro are also related to HIR or its enhancement in vivo.