6 Base PCR to Build a Superior Genetic Detection System

描述（由申请人提供）：虽然，所有生命系统的遗传信息的存储来源于两个碱基对（A:T和G:C）的组织串，但其他核酸碱基和碱基对在化学上是可能的。我们已经证明，两个额外的碱基编码第三个碱基对可以应用于PCR与自然的四碱基对，两碱基对系统。本项目将重点利用这个新发现的六碱基PCR系统来推进和简化高多重遗传分析的核苷酸连接系统。三碱基对PCR技术将用于遗传分析系统，该系统首先使用连接特异性配对序列特异性引物集，然后使用PCR扩增这些连接集。使用三个碱基对提供的额外复杂性将允许提出的分析系统的产品通过室温杂交进行分析。与单独使用天然DNA相比，这种分析将实现更高的复杂性和更好的区分。目的是简化和推进遗传分析技术与六碱基PCR，以降低成本，提高鲁棒性和准确性。如果这可以在该项目的第一阶段证明，其他化学物质将受益于六碱基PCR系统，包括适体生产和DNA标记策略。

DESCRIPTION (provided by applicant): Although, the storage of genetic information for all living systems is derived from the organizational string of just two base pairs (A:T and G:C), additional nucleic acid bases and base-pairs are chemically possible. We have shown that two additional bases encoding a third base pair can be applied to PCR along with the I four-base, two-base-pair system of nature. This project will focus on using this newly discovered six-base PCR system to advance and simplify ligonucleotide ligation systems for highly multiplexed genetic analysis. The three-base-pair PCR technology will be used in a genetic analysis system which first uses ligation to specifically couple sequence-specific primer sets and then PCR to amplify those ligated sets. The additional complexity provided by using three base pairs will allow the products of the proposed analysis system to be analyzed by room temperature hybridization. This analysis will achieve higher complexity and better discrimination than that possible with natural DNA alone. The goal is to simplify and advance genetic analysis technology with six-base PCR in order to lower cost and increase robustness and accuracy. If this can be demonstrated in Phase I of this project, additional chemistries should benefit from a six-base PCR system including aptamer production and DNA tagging strategies.