MOLECULAR BIOLOGY AND TISSUE ENGINEERING CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 现有的分子生物学和组织培养实验室（MBL/TCL）的核心单位，在促进各种教师和研究人员在得克萨斯州南方大学（大津）的研究活动发挥了关键作用。MBL/TCL的主要目的是通过提供资源和技术支持以及这些领域的实践培训，加强细胞和分子水平的研究调查。为了提高生物医学研究竞技场的竞争力，支持新的学术目标，并符合后基因组技术的进步，现有的MBL/TCL需要继续支持，以加强和扩大现有的资源。该设施的增强将使MBL/TCL核心能够提供关键的后基因组技术和仪器服务。 三维（3-D）组织培养技术最近彻底改变了生物医学研究，特别是在组织移植，癌症研究和药物设计领域。这种3-D组织培养技术提供了一种新的体外组织模型系统，用于了解基因、蛋白质和受体表达方面的细胞-细胞相互作用。随着越来越多的研究人员转向3D细胞培养，开发这种能力的需求是及时的，因为基因表达和其他生物活动的模式更接近地反映了生命系统中发生的事情。组织工程学的最新发展清楚地表明，二维平面细胞层和复杂的三维组织之间存在许多差异。MBL/TCL设施的扩建涉及增加新的组织工程（TE）组件。该组件将提供培训，技术支持和基础设施，以培养复杂的3-D组织等效架构，该架构模拟体内条件，用于研究人类健康和疾病状态下的基因/蛋白质表达和细胞-细胞相互作用。因此，本项目的目的是保持资金，以加强和扩大现有的MBL/TCL核心设施与新的TE技术，形成分子生物学和组织工程（MBTE）核心设施。组织工程组成部分的整合将补充现有的MBL/TCL核心设施，并加强其服务能力，在使用整个动物进行体内研究之前进行先进的体外研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The existing Molecular Biology and Tissue Culture Laboratory (MBL/TCL) core unit has played a key role in fostering research activities of various faculty and research investigators at Texas Southern University (TSU). The main purpose of the MBL/TCL was to enhance research investigations at the cellular and molecular level by providing resources and technical support, as well as hands-on-training in these areas. In order to increase competitiveness in the biomedical research arena, support new academic goals, and conform to post-genomic technological advances, the existing MBL/TCL requires continued support to enhance and expand the available resources. Enhancement of this facility will enable the MBL/TCL core to provide critical post-genomic technical and instrumentation services. The 3-dimensional (3-D) tissue culture technology has recently revolutionized biomedical research, particularly in the areas of tissue grafting, cancer research, and drug design. This 3-D tissue culture technology provides a novel in vitro tissue model system for understanding cell-cell interactions with respect to gene, protein, and receptor expression. The need for developing such a capability is timely as more researchers are increasingly turning to 3-D cell culture since patterns of gene expression and other biological activities more closely mirror what happens in a living system. Recent developments in tissue engineering clearly suggest that there are numerous differences between a 2-D flat layer of cells and a complex 3-D tissue. Expansion of the MBL/TCL facility involves the addition of a new Tissue Engineering (TE) component. This component will provide the training, technical support, and infrastructure to grow the complex 3-D tissue-equivalent architecture, which simulates in vivo, conditions for studying gene/protein expression and cell-cell interactions in human health and disease states. Therefore, the purpose of this project is to maintain funds to enhance, and expand the existing MBL/TCL core facility with a new TE technology to form the Molecular Biology and Tissue Engineering (MBTE) Core Facility. Integration of a tissue engineering component will complement the existing MBL/TCL core facility and strengthen its service capability to conduct advanced in vitro studies before proceeding to in vivo studies using whole animals.