Acquisition of Shared Thermoneutral Rodent Housing Resources

项目摘要/摘要 需要在中温条件下，即在环境中，安置和饲养实验室啮齿动物 能量消耗被限制在维持基础代谢率所需的温度是 越来越多的人认识到这对取得严谨的研究成果至关重要。温度效应的证据 一系列的生理功能和病理，包括能量消耗、免疫功能和 反应、血压、心率、睡眠、食物摄入量、肿瘤生长和耐药性等都有所提高 认识到有必要将热中性因素纳入研究设计，以提高研究的有效性 研究结果及其翻译，以了解和增进人类健康。以适应最近和正在进行的 在温差条件下对小鼠进行生物医学研究的校园扩展能力， 肯塔基大学(英国)提议在其最新的隔间建立一个热中和套房。 最近建造的健康肯塔基研究大楼(HKRB)。3000平方英尺英国《金融时报》HKRB Varium支持一个 265,000平方英尺英国《金融时报》六层楼的最先进的生物医学研究设施于2018年开放，毗邻 相邻的两座现代化研究大楼中的动物设施。这项设备应用的长期目标是 加强和现代化由英国实验室部门管理的动物研究支持业务 动物资源(DLAR)通过配备三个房间的热中性套房(970平方英尺)英国《金融时报》空间)与 专用的高级温差笼子。拟议的套房将由法援署运营，并由 负责研究核心设施的协理副总裁将实现具体目标，使 获得六个移动融合区IVC笼子在中温条件下繁殖和安置小鼠群体 系统将安装在385平方英尺的空间内英国《金融时报》毗邻两个房间的空间，配备用于在 热中和研究条件。向美国国家卫生研究院提出的这项请求将补充单独收购的 热中和实验设备将合并到两个相邻的房间，但不是这笔资金的一部分 请求。建成后的套件将提升DLAR为NIH资助的调查人员提供的研究支持业务 世卫组织的研究需要进入三个Power Science Inc.型号RIS70SD啮齿动物实验室 支持行为研究和四个适合进行昼夜节律研究的时钟实验室， 其中一项已经到位。HKRB间隔室是一座现代化的高端设施。这次设施升级将 实现新的研究效率，并为研究目标要求更高的研究人员提供更严格的要求 通过综合研究支持行动，仔细校准环境条件。解决这个问题 对尖端移动式动物收容所的需求将影响美国国立卫生研究院资助的各种心脏研究 心律失常，血管性痴呆和神经变性，骨骼肌肥大，代谢性疾病，胎儿 编程，以及更多，以产生更严格的研究结果。这些地区代表了英国最大的 由高生产率和协作性的教员开展的重要领域的研究活动。

PROJECT SUMARY/ABSTRACT The need to house and maintain laboratory rodents under conditions of thermoneutrality, i.e., at the ambient temperature at which energy expenditure is limited to that required to maintain basal metabolic rate, is increasingly recognized as critical to achieving rigorous research outcomes. Evidence of temperature effects on a vast array of physiological functions and pathologies spanning energy expenditure, immune function and response, blood pressure, heart rate, sleep, food intake, tumor growth and resistance, and more have raised awareness of the need to factor thermoneutrality into research designs to enhance the validity of research findings and their translation to understand and enhance human health. To accommodate recent and ongoing campus expansion of research capabilities for conducting biomedical studies in mice under thermoneutrality, the University of Kentucky (UK) proposes to establish a Thermoneutrality Suite in its newest vivarium in the recently constructed Healthy Kentucky Research Building (HKRB). The 3,000 sq. ft. HKRB vivarium supports a 265,000 sq. ft. six-floor state-of-the-art biomedical research facility that opened in 2018 and is contiguous to animal facilities in two adjacent modern research buildings. The long-term goal of this equipment application is to enhance and modernize animal research support operations managed by the UK Division of Laboratory Animal Resources (DLAR) by equipping a three-room Thermoneutrality Suite (970 sq. ft. of space) with dedicated advanced thermoneutrality caging. The proposed suite, to be operated by DLAR and overseen by the Associate Vice President for Research Core Facilities, will achieve the specific aim to permit both the breeding and housing of mouse colonies at thermoneutrality by acquiring six mobile Solace Zone IVC caging systems to be installed in a 385 sq. ft. space adjoining two rooms equipped for experimentation under thermoneutral study conditions. This request to the NIH will complement acquisition of separately acquired thermoneutral experimental equipment to be consolidated in two adjacent rooms, but not part of this funding request. The completed suite will elevate DLAR’s research-supporting operations for NIH-funded investigators who studies require access to three Power Scientific Inc. model RIS70SD rodent experimental chambers to support behavioral studies and four Actimetrics Clocklab Chambers suitable for performing circadian studies, one of which is already in place. The HKRB vivarium is a modern, high-end facility. This facilities upgrade will enable new research efficiencies and provide enhanced rigor to researchers whose study objectives require carefully calibrated environmental conditions through an integrated research support operation. Addressing this need for cutting-edge mobile animal housing units will impact diverse NIH-funded studies spanning cardiac arrythmias, vascular dementia and neurodegeneration, skeletal muscle hypertrophy, metabolic disease, fetal programming, and more to produce more rigorous study outcomes. These areas represent some of UK’s most prominent areas of research activity conducted by a highly productive and collaborative faculty.