Colorado Gene Array Core

科罗拉多基因阵列核心将集中于创建数据库，可用于开发 小鼠和大鼠脑的表达QTL（eQTL）图谱。我们目前正在扩大大脑的数据库 BxD R1小鼠品系的雄性和雌性的基因表达谱。拟议的研究将 从31个R1品系的BxH/HxB大鼠的脑和R1品系的BxH/HxB大鼠的脑中产生基因表达谱的类似数据库。 祖先品系（分别为雄性和雌性），以及大鼠品系的微卫星和SNP基因分型 将与田纳西大学的RobWilliams博士合作进行。这些信息将使 用于大鼠脑基因表达的eQTL定位以及大鼠脑饮酒的QTL定位 菌株当与复杂性状的QTL分析相结合时，eQTL的产生有助于 识别这些性状的候选基因，核心将使用这一程序和生成的数据 以确定导致大鼠焦虑和其他酒精相关行为的候选基因。的 脑基因表达数据的可用性和小鼠和大鼠脑转录组的eQTL定位将 为酗酒调查者提供了一个新颖独特的资源。核心也将执行基因 表达分析和定量RT-PCR为合作的INIA研究人员，并提供访问 标准化、统计分析和eQTL搜索的方法。

The Colorado Gene Array Core will focus on the creation of databases that can be used for developing expression QTL (eQTL) maps for mouse and rat brain. We are currently expanding the database of brain gene expression profiles for males and females of the BxD Rl mouse strains. The proposed studies will generate a similar database of gene expression profiles from brains of 31 Rl strains of BxH/HxB rats and the progenitor strains (males and females separately), and microsatellite and SNP genotyping of the rat strains will be performed in collaboration with Dr. RobWilliams, University of Tennessee. This information will allow for eQTL mapping for rat brain gene expression as well as mapping of QTLs for alcohol drinking in the rat strains. When combined with QTL analysis of complex traits, the generation of eQTLs facilitates the identification of candidate genes for these traits, and the Core will use this procedure and the generated data to identify candidate genes that contribute to anxiety and other alcohol related behaviors in the rats. The availability of brain gene expression data and eQTL mapping of the mouse and rat brain transcriptome will produce a novel and unique resource for use by alcoholism investigators. The Core will also perform gene expression analysis and quantitative RT-PCR for collaborating INIA investigators, and provide access to methodology for normalization, statistical analysis and eQTL searches of the generated data.