Multiplexed cell for high-throughput FT-ICR MS

描述（由申请人提供）：本提案的目的是开发一种通过傅里叶变换离子回旋共振（FT-ICR）质谱法对生物分子进行高通量质量分析的新技术。FT-ICR质谱仪在所有类型的质量分析仪中提供最高的质量准确度和分辨率，但分析速度是其缺点。所提出的技术解决了这个问题。其主要特点是一个新的FT-ICR分析单元，它在设计和操作原理上不同于任何其他分析单元。在新的细胞中，离子回旋运动的激发和检测功能在不同的隔间之间分离。此外，检测发生在由同轴检测电极组成的隔室中。这样的设计允许在回旋加速器频率的倍数上执行检测，因此增加了分析的速度，而没有灵敏度的损失和与常规多电极池相反的其他有害影响。通过具有一个用于激发离子回旋运动的隔室和多个用于同时检测不同离子群的回旋运动的隔室，进一步提高了分析速度。该技术可以使FT-ICR的分析速度提高一个数量级。

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a novel technology for high throughput mass analysis of biomolecules by Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. FT-ICR mass spectrometers offer the highest mass accuracy and resolution among all types of mass analyzers, but speed of the analysis is their drawback. The proposed technology addresses this issue. The main its feature is a new FT-ICR analyzer cell which differs from any other analyzer cell in its design and operation principles. In the new cell, functions of the excitation of the ion cyclotron motion and its detection are separated between different compartments. Further, detection takes place in the compartment composed of coaxial detecting electrodes. Such design allows performing detection on a multiple of the cyclotron frequency, thus increasing the speed of the analysis, without loss of sensitivity and other detrimental effects as opposed to conventional multi-electrode cells. The speed of analysis is further increased by having one compartment for excitation of the ion cyclotron motion and several compartments for the simultaneous detection of the cyclotron motion of different ion populations. This technology can result in up to an order of the magnitude increase of the analysis speed in FT-ICR.