Modernization of 3-dimensional printing capabilities at the Aquatic Germplasm and Genetic Resource Center

项目摘要/摘要： 水生物种已被公认为生物医学研究和周围实验室的有价值的模型 世界每年生产数以万计的突变、基因敲除、基因编辑和转基因品系。 建立高效可靠的种质资源库是保存冬小麦遗传资源的关键 对推进生物医学研究至关重要的水生生物。然而，普遍缺乏重复性 在冷冻保存中产生了一个显著的障碍，造成了丢失从 数十亿美元的研究投资。有两个主要问题：第一，尽管协议已经 实验室是通过基础研究建立的，由于缺乏基础研究，实验室经常生产低质量的样品 价格实惠且可靠的硬件，以实现标准化的生产路径。第二，没有有效的 在系统一级提供质量管理的方法，包括预防 缺陷，并进行质量控制，以消除劣质产品。尽管存在一些商业选择，但大多数 当种质库不是重点时，实验室无法购买昂贵的设备 义务。在之前的R24项目中，我们与斑马鱼国际资源中心(Zirc)合作 为他们建立强大的现场能力，作为中心设施。我们还开发了机械设备 通过3-D打印和微制造，在Zirc开始标准化工作。我们正在扩大这一范围 技术倡议，广泛应用于全面生产和质量所需的各个步骤 通过开发多种类型的实用的新科学硬件来管理路径， 价格低廉、可标准化、可移植和可定制，并可作为用户驱动的公共服务提供 通过开放硬件为数千个实验室提供工具箱。在过去的10年里，AGGRC，一个共享的- Use Facility，已成为生产用于低温保存和储存库的开放式硬件的领导者 发展。到目前为止，我们主要将我们的工作限制在消费级设备上，以确保 作为可打印计算机文件分发的硬件将向所有用户提供，无论其技能水平如何。 尽管我们已经具备了基于长丝(例如塑料)的3D打印技术，但我们仍非常需要 大幅提高我们使用光固化树脂和金属进行3D打印的能力。现代化的设备是 对这一领域持续的强大影响力至关重要。例如，我们采用树脂印刷作为一种 替代昂贵和耗时的微细加工(例如，光刻)，并且需要金属 打印与树脂一起工作，以制造定制电极和传感器等工具。实现我们的现代化 这些能力将极大地简化和改进操作流程和程序，并将提供更多- 需要获得先进的技术设备，以支持当前、快速发展和新兴的 研究项目。这将实现新的和先进的方法，提供创新的技术解决方案， 并使我们机构内外的用户社区和多个研究项目和研究人员受益。

PROJECT SUMMARY/ABSTRACT: Aquatic species have become accepted as valuable models in biomedical research, and laboratories around the world produce tens of thousands of mutant, knockout, gene-edited and transgenic lines each year. Establishment of efficient and reliable germplasm repositories is critical for preservation of genetic resources of aquatic organisms that are vital to advancing biomedical research. However, a pervasive lack of reproducibility in cryopreservation creates a significant barrier, posing a great risk of losing valuable lines developed from billions of dollars of research investment. There are two major problems: first, although protocols have been established through basic research, laboratories often produce low-quality samples because of a lack of affordable and reliable hardware for a standardized production pathway. Second, there are no effective approaches to provide quality management at a system level, including quality assurance for prevention of defects, and quality control for elimination of inferior products. Although some commercial options exist, most laboratories are not able to purchase expensive equipment when germplasm banking is not a focus or obligation. In previous R24 projects we worked with the Zebrafish International Resource Center (ZIRC) to establish strong on-site capabilities for them as a central facility. We also developed mechanical devices through 3-D printing and microfabrication to begin standardization efforts at ZIRC. We are expanding this technology initiative for widespread use across the steps required for comprehensive production and quality management pathways by development of multiple types of new scientific hardware that are practical, inexpensive, standardizable, portable, and customizable, and can be made available as user-driven public toolboxes through open hardware for thousands of laboratories. Over the past 10 years the AGGRC, a shared- use facility, has become a leader in production of open hardware for cryopreservation and repository development. To date, we have primarily restricted our work to consumer-level equipment to ensure that hardware distributed as printable computer files would be available to all users regardless of skill level. Although we are well-equipped for filament-based (e.g., plastic) 3-D printing, we have great need to substantially improve our ability to 3-D print with photocurable resins and metal. Modernized equipment is essential for a sustained powerful influence in this field. For example, we have adopted resin printing as an alternative to expensive and time-consuming microfabrication (e.g., photolithography), and require metal printing to work with resins to fabricate tools such as custom electrodes and sensors. Modernizing our capabilities will greatly streamline and improve operating processes and procedures, and it will provide much- needed access to technologically advanced equipment to support current, rapidly evolving, and emerging research programs. This will enable new and advanced approaches, offer innovative technological solutions, and benefit user communities and multiple research projects and investigators at our institution and beyond.